ArticleBiophysical journal2001
Robust, high-resolution, whole cell patch-clamp capacitance measurements using square wave stimulation.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Article
- An Electrophysiological Approach to Measure Changes in the Membrane Surface Potential in Real Time.Biophysical journal · 2020Article
- Optical Tracking of Nanometer-Scale Cellular Membrane Deformation Associated with Single Vesicle Release.ACS sensors · 2019Article
- Article
- Article
- Reconstruction of membrane current by deconvolution and its application to membrane capacitance measurements in cardiac myocytes.PloS one · 2017Article
- Non-Faradaic Electrochemical Detection of Exocytosis from Mast and Chromaffin Cells Using Floating-Gate MOS Transistors.Scientific reports · 2015Article
- High resolution electrophysiological techniques for the study of calcium-activated exocytosis.Biochimica et biophysica acta · 2012Review
- Zf-and-Hsys-based Cm measurement under the whole-cell patch-clamp recording.Pflugers Archiv : European journal of physiology · 2009Article
- Soft trapping and manipulation of cells using a disposable nanoliter biochamber.Biophysical journal · 2006Article
- Effect of input resistance voltage-dependency on DC estimate of membrane capacitance in cardiac myocytes.Biophysical journal · 2005Article
- Determination of cell capacitance using the exact empirical solution of partial differential Y/partial differential Cm and its phase angle.Biophysical journal · 2004Article
- A large pool of releasable vesicles in a cortical glutamatergic synapse.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Intracellular patch electrochemistry: regulation of cytosolic catecholamines in chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
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Authors and funding
3 authors.
Funding
Abstract
High-resolution, whole cell capacitance measurements are usually performed using sine wave stimulation using a single frequency or a sum of two frequencies. We present here a high-resolution technique for whole-cell capacitance measurements based on square-wave stimulation. The square wave represents a sum of sinusoidal frequencies at odd harmonics of the base frequency, the amplitude of which is highest for the base frequency and decreases as the frequency increases. The resulting currents can be analyzed by fitting the current relaxations with exponentials, or by a phase-sensitive detector technique. This method provides a resolution undistinguishable from that of single-frequency sine wave stimulation, and allows for clear separation of changes in capacitance, membrane conductance, and access resistance. In addition, it allows for the analysis of more complex equivalent circuits as associated with the presence of narrow fusion pores during degranulation, tracking many equivalent circuit parameters simultaneously. The method is insensitive to changes in the reversal potential, pipette capacitance, or widely varying cell circuit parameters. It thus provides important advantages in terms of robustness for measuring cell capacitances, and allows analysis of complicated changes of the equivalent circuits.
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