ArticleThe Biochemical journal2001
Knockout of mouse beta 1,4-galactosyltransferase-1 gene results in a dramatic shift of outer chain moieties of N-glycans from type 2 to type 1 chains in hepatic membrane and plasma glycoproteins.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 57 citations in OpenAlex.
- Identification of a free-N-glycan with difucosylated type-1 lactosamine in cancerous tissue and its validation for cancer diagnosis by urinary testing.Scientific reports · 2025Article
- Aberration of Serum and Tissue N-Glycans in Mouse β1,4-GalT1 Y286L Mutant Variants.Glycoconjugate journal · 2020Article
- β1,4-galactosyltransferase-I protects chondrocytes against TNF-induced apoptosis by blocking the TLR4 signaling pathway.American journal of translational research · 2019Article
- Challenge to the suppression of tumor growth by the β4-galactosyltransferase genes.Proceedings of the Japan Academy. Series B, Physical and biological sciences · 2015Review
- Glycosylation-related genes in NS0 cells are insensitive to moderately elevated ammonium concentrations.Journal of biotechnology · 2014Article
- The Angelman syndrome protein Ube3a/E6AP is required for Golgi acidification and surface protein sialylation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Mice overexpressing β-1,4-Galactosyltransferase I are resistant to TNF-induced inflammation and DSS-induced colitis.PloS one · 2013Article
- Disruption of Smad4 expression in T cells leads to IgA nephropathy-like manifestations.PloS one · 2013Article
- Elevated expression of β1,4-galactosyltransferase-I in cartilage and synovial tissue of patients with osteoarthritis.Inflammation · 2012Article
- Tumor necrosis factor-α up-regulates the expression of β1,4-galactosyltransferase-I in human fibroblast-like synoviocytes.Inflammation · 2011Article
- β1,4-galactosyltransferase-I in synovial tissue of collagen-induced rat model of rheumatoid arthritis.Clinical and experimental medicine · 2011Article
- β1,4-Galactosyltransferase-I contributes to the inflammatory processes in synovial tissue of patients with rheumatoid arthritis.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2010Article
- Genomic implications of H(2)O (2) for cell proliferation and growth of Caco-2 cells.Digestive diseases and sciences · 2007Article
- Expression of beta-1,4-galactosyltransferase-I in rat during inflammation.Inflammation · 2007Article
- The congenital disorders of glycosylation: a multifaceted group of syndromes.NeuroRx : the journal of the American Society for Experimental NeuroTherapeutics · 2006Review
- Impairment of skin wound healing in beta-1,4-galactosyltransferase-deficient mice with reduced leukocyte recruitment.The American journal of pathology · 2004Article
- Keratan sulfate biosynthesis.IUBMB life · 2002Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To understand the contribution of beta 1,4-galactosyltransferase (beta 4Gal-T)-1 to galactosylation in vivo, N-glycans of hepatic membrane glycoproteins and plasma glycoproteins from beta 4Gal-T1 wild-type (beta 4Gal-T1(+/+)) and beta 4Gal-T1 knockout mice were compared. Unexpectedly, glycoproteins from the knockout mice were found to express considerable amounts of sialylated, galactosylated N-glycans. A striking contrast was that galactose residues were largely beta 1,4-linked to GlcNAc residues in the beta 4Gal-T1(+/+) mouse glycans but beta 1,3-linked in the knockout mouse glycans, thus resulting in the shift of the backbone structure from type 2 chain (Gal beta 1-->4GlcNAc) to type 1 chain (Gal beta 1-->3GlcNAc). Detailed analysis of plasma glycoproteins revealed that the expression of sialyl linkage in N-glycans was shifted from the Sia alpha 2-->6Gal to the Sia alpha 2-->3Gal, and oversialylated type 1 chains were, remarkably, found in the knockout mouse glycans. Thus beta 4Gal-T1 deficiency was primarily compensated for by beta1,3-galactosyltransferases, which resulted in different sialyl linkages being formed on the outer chains and altered backbone structures, depending on the acceptor specificities of sialyltransferases. These results suggest that beta 4Gal-T1 in mouse liver plays a central role in the synthesis of type 2 chain and is also involved in the regulation of sialylation of N-glycans. The knockout mice may prove useful in investigation of the mechanism which regulates the tissue-dependent terminal glycosylation.
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