ArticleJournal of virology2001
Use of patient-derived human immunodeficiency virus type 1 integrases to identify a protein residue that affects target site selection.
Article in Journal of virology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Unraveling the palindromic and nonpalindromic motifs of retroviral integration site sequences by statistical mixture models.Genome research · 2023Article
- Review
- Sites of retroviral DNA integration: From basic research to clinical applications.Critical reviews in biochemistry and molecular biology · 2016Review
- Design, Synthesis, and Biological Evaluation of 1,2-Dihydroisoquinolines as HIV-1 Integrase Inhibitors.ACS medicinal chemistry letters · 2015Article
- Integrase residues that determine nucleotide preferences at sites of HIV-1 integration: implications for the mechanism of target DNA binding.Nucleic acids research · 2014Article
- HIV-2 integrase polymorphisms and longitudinal genotypic analysis of HIV-2 infected patients failing a raltegravir-containing regimen.PloS one · 2014Article
- piggyBac transposase tools for genome engineering.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Alternative nucleophilic substrates for the endonuclease activities of human immunodeficiency virus type 1 integrase.Virology · 2012Article
- Evaluation of a system to screen for stimulators of non-specific DNA nicking by HIV-1 integrase: application to a library of 50,000 compounds.Antiviral chemistry & chemotherapy · 2011Article
- Integration specificity of LTR-retrotransposons and retroviruses in the Drosophila melanogaster genome.Virus genes · 2011Article
- Structural biology of retroviral DNA integration.Virology · 2011Review
- Localization of ASV integrase-DNA contacts by site-directed crosslinking and their structural analysis.PloS one · 2011Article
- Chemistry and structure-activity relationship of the styrylquinoline-type HIV integrase inhibitors.Molecules (Basel, Switzerland) · 2010Review
- Specificity of LTR DNA recognition by a peptide mimicking the HIV-1 integrase {alpha}4 helix.Nucleic acids research · 2009Article
- HIV-1 Integrase-DNA Recognition Mechanisms.Viruses · 2009Article
- Biochemical and virological analysis of the 18-residue C-terminal tail of HIV-1 integrase.Retrovirology · 2009Article
- Structural basis for functional tetramerization of lentiviral integrase.PLoS pathogens · 2009Article
- Defining the DNA substrate binding sites on HIV-1 integrase.Journal of molecular biology · 2009Article
- Division of labor within human immunodeficiency virus integrase complexes: determinants of catalysis and target DNA capture.Journal of virology · 2005Article
- Genetic analyses of conserved residues in the carboxyl-terminal domain of human immunodeficiency virus type 1 integrase.Journal of virology · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
To identify parts of retroviral integrase that interact with cellular DNA, we tested patient-derived human immunodeficiency virus type 1 (HIV-1) integrases for alterations in the choice of nonviral target DNA sites. This strategy took advantage of the genetic diversity of HIV-1, which provided 75 integrase variants that differed by a small number of amino acids. Moreover, our hypothesis that biological pressures on the choice of nonviral sites would be minimal was validated when most of the proteins that catalyzed DNA joining exhibited altered target site preferences. Comparison of the sequences of proteins with the same preferences then guided mutagenesis of a laboratory integrase. The results showed that single amino acid substitutions at one particular residue yielded the same target site patterns as naturally occurring integrases that included these substitutions. Similar results were found with DNA joining reactions conducted with Mn(2+) or with Mg(2+) and were confirmed with a nonspecific alcoholysis assay. Other amino acid changes at this position also affected target site preferences. Thus, this novel approach has identified a residue in the central domain of HIV-1 integrase that interacts with or influences interactions with cellular DNA. The data also support a model in which integrase has distinct sites for viral and cellular DNA.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.