ArticleBritish journal of cancer2001
Analysis of differentially expressed genes in human hepatocellular carcinoma using suppression subtractive hybridization.
Article in British journal of cancer, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 82 citations in OpenAlex.
- OncomiR-181a promotes carcinogenesis by repressing the extracellular matrix proteoglycan decorin in hepatocellular carcinoma.BMC gastroenterology · 2024Article
- Identification of PTK2 as an adverse prognostic biomarker in breast cancer by integrated bioinformatics and experimental analyses.Frontiers in molecular biosciences · 2022Article
- Breast Cancer-Derived Microvesicles Are the Source of Functional Metabolic Enzymes as Potential Targets for Cancer Therapy.Biomedicines · 2021Article
- Bone Marrow-Derived Mesenchymal Stem Cells Differentially Affect Glioblastoma Cell Proliferation, Migration, and Invasion: A 2D-DIGE Proteomic Analysis.BioMed research international · 2021Article
- Protective Role of Decorin in Primary Hepatocellular Carcinoma.Frontiers in oncology · 2020Article
- Mechanism of Inactivation of Ornithine Aminotransferase by (1Journal of the American Chemical Society · 2019Article
- Decorin as a prognostic biomarker in patients with malignant peripheral nerve sheath tumors.Oncology letters · 2019Article
- Overexpressed pepsinogen C is associated with poor prognosis in human hepatocellular carcinoma: a tissue microarray study.Cancer management and research · 2019Article
- Adenovirus-mediated decorin expression induces cancer cell death through activation of p53 and mitochondrial apoptosis.Oncotarget · 2017Article
- Selective Targeting by a Mechanism-Based Inactivator against Pyridoxal 5'-Phosphate-Dependent Enzymes: Mechanisms of Inactivation and Alternative Turnover.Biochemistry · 2017Article
- Article
- Ornithine Aminotransferase, an Important Glutamate-Metabolizing Enzyme at the Crossroads of Multiple Metabolic Pathways.Biology · 2017Review
- Focal Adhesion Kinase: Insight into Molecular Roles and Functions in Hepatocellular Carcinoma.International journal of molecular sciences · 2017Review
- Pepsinogen C expression, regulation and its relationship with cancer.Cancer cell international · 2017Review
- High expression of apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) in human cholangiocarcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Proteoglycans in liver cancer.World journal of gastroenterology · 2016Review
- Suppression of Hepatocellular Carcinoma by Inhibition of Overexpressed Ornithine Aminotransferase.ACS medicinal chemistry letters · 2015Article
- Ornithine aminotransferase versus GABA aminotransferase: implications for the design of new anticancer drugs.Medicinal research reviews · 2015Review
- Small nucleolar RNA 113-1 suppresses tumorigenesis in hepatocellular carcinoma.Molecular cancer · 2014Article
- Decorin interferes with platelet-derived growth factor receptor signaling in experimental hepatocarcinogenesis.The FEBS journal · 2013Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genetic basis of hepatocellular carcinoma (HCC) has not yet been fully understood. Although various methods have been developed to detect differentially expressed genes in malignant diseases, efficient analysis from clinical specimens is generally difficult to perform due to the requirement of a large amount of samples. In the present study, we analysed differentially expressed genes with a small amount of human HCC samples using suppression subtractive hybridization (SSH). Total RNA were obtained from the hepatitis C virus-associated HCC and adjacent non-HCC liver tissues. cDNA was synthesized using modified RT-PCR, and then tester cDNA was ligated with 2 different kinds of adaptors and hybridized with an excess amount of driver cDNA. Tester specific cDNA was obtained by suppression PCR and the final PCR product was subcloned and sequenced. We identified 7 known genes (focal adhesion kinase, deleted in colon cancer, guanine binding inhibitory protein alpha, glutamine synthetase, ornithine aminotransferase, M130, and pepsinogen C) and 2 previously unknown genes as being overexpressed in HCC, and 1 gene (decorin) as suppressed in HCC. Quantitative analysis of gene expression using quantitative RT-PCR demonstrated the differential expression of these genes in the original and other HCC samples. These findings demonstrated that it is possible to identify the previously unknown, differential gene expression from a small amount of clinical samples. Information about such alterations in gene expression could be useful for elucidating the genetic events in HCC pathogenesis, developing the new diagnostic markers, or determining novel therapeutic targets.
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