ArticleGenes & development2001
The set1Delta mutation unveils a novel signaling pathway relayed by the Rad53-dependent hyperphosphorylation of replication protein A that leads to transcriptional activation of repair genes.
Article in Genes & development, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 43 citations in OpenAlex.
- Insights into the recruitment of the H3K4me3 reader Spp1 by the meiotic double-strand break protein Mer2.Nucleic acids research · 2026Article
- Ready, SET, Go: Post-translational regulation of the histone lysine methylation network in budding yeast.The Journal of biological chemistry · 2021Review
- Regulation of alternative polyadenylation in the yeast Saccharomyces cerevisiae by histone H3K4 and H3K36 methyltransferases.Nucleic acids research · 2020Article
- Article
- Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae.Experimental cell research · 2015Article
- TopBP1/Dpb11 binds DNA anaphase bridges to prevent genome instability.The Journal of cell biology · 2014Article
- Spp1 at the crossroads of H3K4me3 regulation and meiotic recombination.Epigenetics · 2013Review
- Mutagenesis of pairwise combinations of histone amino-terminal tails reveals functional redundancy in budding yeast.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Methylated H3K4, a transcription-associated histone modification, is involved in the DNA damage response pathway.PLoS genetics · 2010Article
- Origin recognition complex (ORC) mediates histone 3 lysine 4 methylation through cooperation with Spp1 in Saccharomyces cerevisiae.The Journal of biological chemistry · 2008Article
- Dumpy-30 family members as determinants of male fertility and interaction partners of metal-responsive transcription factor 1 (MTF-1) in Drosophila.BMC developmental biology · 2008Article
- A role for Chd1 and Set2 in negatively regulating DNA replication in Saccharomyces cerevisiae.Genetics · 2008Article
- Subtelomeric proteins negatively regulate telomere elongation in budding yeast.The EMBO journal · 2006Article
- Inactivation of Ku-mediated end joining suppresses mec1Delta lethality by depleting the ribonucleotide reductase inhibitor Sml1 through a pathway controlled by Tel1 kinase and the Mre11 complex.Molecular and cellular biology · 2005Article
- Role of Dot1-dependent histone H3 methylation in G1 and S phase DNA damage checkpoint functions of Rad9.Molecular and cellular biology · 2005Article
- The Set1 methyltransferase opposes Ipl1 aurora kinase functions in chromosome segregation.Cell · 2005Article
- A haploid-specific transcriptional response to irradiation in Saccharomyces cerevisiae.Nucleic acids research · 2005Article
- Set1 is required for meiotic S-phase onset, double-strand break formation and middle gene expression.The EMBO journal · 2004Article
- The essential WD repeat protein Swd2 has dual functions in RNA polymerase II transcription termination and lysine 4 methylation of histone H3.Molecular and cellular biology · 2004Article
- The number of vertebrate repeats can be regulated at yeast telomeres by Rap1-independent mechanisms.The EMBO journal · 2003Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SET domain proteins are present in chromosomal proteins involved in epigenetic control of transcription. The yeast SET domain protein Set1p regulates chromatin structure, DNA repair, and telomeric functions. We investigated the mechanism by which the absence of Set1p increases DNA repair capacities of checkpoint mutants. We show that deletion of SET1 induces a response relayed by the signaling kinase Rad53p that leads to the MEC1/TEL1-independent hyperphosphorylation of replication protein A middle subunit (Rfa2p). Consequently, the binding of Rfa2p to upstream repressing sequences (URS) of repair genes is decreased, thereby leading to their derepression. Our results correlate the set1Delta-dependent phosphorylation of Rfa2p with the transcriptional induction of repair genes. Moreover, we show that the deletion of the amino-terminal region of Rfa2p suppresses the sensitivity to ultraviolet radiation of a mec3Delta checkpoint mutant, abolishes the URS-mediated repression, and increases the expression of repair genes. This work provides an additional link for the role of Rfa2p in the regulation of the repair capacity of the cell and reveals a role for the phosphorylation of Rfa2p and unveils unsuspected connections between chromatin, signaling pathways, telomeres, and DNA repair.
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