ArticlePlant physiology2001
Superoxide production by plant homologues of the gp91(phox) NADPH oxidase. Modulation of activity by calcium and by tobacco mosaic virus infection.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 146 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
146 citing papers in PubMed, 608 citations in OpenAlex.
- Foliar GR24 and γ-aminobutyric acid modulate redox signaling, root-system architecture, and mineral homeostasis in tellurite-stressed wheat (Plant signaling & behavior · 2026Article
- Characterization of the Differential Tolerance of TwoPlants (Basel, Switzerland) · 2026Article
- Regulation of aluminum induced oxidative stress in Brassica via roles of sulfur metabolites and antioxidant responses mediated by a melatonin selenium nanocomposite.BMC plant biology · 2025Article
- Article
- Comparative insights into soybean and other oilseed crops' defense mechanisms againstFrontiers in plant science · 2025Review
- The LRR receptor-like kinase ALR1 is a plant aluminum ion sensor.Cell research · 2024Article
- Article
- Determination of ROS Generated by Arabidopsis Xanthine Dehydrogenase1 (AtXDH1) Using Nitroblue Tetrazolium (NBT) and 3,3'-Diaminobenzidine (DAP).Methods in molecular biology (Clifton, N.J.) · 2024Article
- Reactive oxygen species derived from NADPH oxidase as signaling molecules regulate fatty acids and astaxanthin accumulation inFrontiers in microbiology · 2024Article
- Regulation of V-ATPase by Jasmonic Acid: Possible Role of Persulfidation.International journal of molecular sciences · 2023Article
- Signal Mediators in the Implementation of Jasmonic Acid's Protective Effect on Plants under Abiotic Stresses.Plants (Basel, Switzerland) · 2023Review
- Molecular Analysis of MgO Nanoparticle-Induced Immunity against Fusarium Wilt in Tomato.International journal of molecular sciences · 2023Article
- Integration of reactive oxygen species and nutrient signalling to shape root system architecture.Plant, cell & environment · 2023Review
- Oxidative Stress Response and Metal Transport in Roots ofPlants (Basel, Switzerland) · 2023Article
- Genome-wide analysis of respiratory burst oxidase homolog (Frontiers in plant science · 2023Article
- Differential regulation of reactive oxygen species in dimorphic chloroplasts of single cell CFrontiers in plant science · 2023Article
- Chitooligosaccharide Maintained Cell Membrane Integrity by Regulating Reactive Oxygen Species Homeostasis at Wounds of Potato Tubers during Healing.Antioxidants (Basel, Switzerland) · 2022Article
- Role of Plasma Membrane NADPH Oxidase in Response to Salt Stress in Cucumber Seedlings.Antioxidants (Basel, Switzerland) · 2022Article
- Differential Impact of Nitric Oxide and Abscisic Acid on the Cellular and Physiological Functioning ofPlants (Basel, Switzerland) · 2022Article
- CaPlant cell reports · 2022Article
86 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genes encoding homologs of the gp91(phox) subunit of the plasma membrane NADPH oxidase complex have been identified in plants and are hypothesized to be a source of reactive oxygen species during defense responses. However, the direct involvement of the gene products in superoxide (O(2)(-)) production has yet to be shown. A novel activity gel assay based on protein fractionation in native or sodium dodecyl sulfate (SDS)-denaturing polyacrylamide gels was developed. In native polyacrylamide gel electrophoresis, one or two major O(2)(-)-producing formazan bands were detected in tomato (Lycopersicum esculentum Mill. cv Moneymaker) and tobacco (Nicotiana tabacum var. Samsun, NN) plasma membranes, respectively. Denaturing fractionation of tomato and tobacco plasma membrane in SDS-polyacrylamide gel electrophoresis, followed by regeneration of the in-gel activity, revealed NADPH-dependent O(2)(-)-producing formazan bands of 106-, 103-, and 80- to 75-kD molecular masses. The SDS and native activity bands were dependent on NADPH and completely inhibited by diphenylene iodonium or CuZn- O(2)(-) dismutase, indicating that the formazan precipitates were due to reduction by O(2)(-) radicals catalyzed by an NADPH-dependent flavin containing enzyme. The source of the plasma membrane activity bands was confirmed by their cross-reaction with antibody prepared from the C terminus of the tomato gp91(phox) homolog. Membrane extracts as well as the in-gel NADPH oxidase activities were stimulated in the presence of Ca(2+). In addition, the relative activity of the gp91(phox) homolog was enhanced in the plasma membrane of tobacco mosaic virus-infected leaves. Thus, in contrast to the mammalian gp91(phox), the plant homolog can produce O(2)(-) in the absence of additional cytosolic components and is stimulated directly by Ca(2+).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.