ArticlePlant physiology2001
The Ca(2+) status of the endoplasmic reticulum is altered by induction of calreticulin expression in transgenic plants.
Article in Plant physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 92 citations in OpenAlex.
- Linking redox and calcium signaling via thioredoxins.Journal of experimental botany · 2026Review
- Article
- Cold-induced calreticulin OsCRT3 conformational changes promote OsCIPK7 binding and temperature sensing in rice.The EMBO journal · 2023Article
- Genome-wide identification, classification, and characterization of lectin gene superfamily in sweet orange (Citrus sinensis L.).PloS one · 2023Article
- HaCRT1 ofFrontiers in plant science · 2020Article
- Proteomic Analysis of Plasmodesmata FromFrontiers in plant science · 2018Article
- Functional Analysis and Marker Development ofFrontiers in plant science · 2017Article
- Molecular characterisation and expression analysis of a novel calreticulin (CRT) gene in the dinoflagellate Prorocentrum minimum.Molecular biology reports · 2015Article
- Insights into animal and plant lectins with antimicrobial activities.Molecules (Basel, Switzerland) · 2015Review
- Overexpression of a Triticum aestivum Calreticulin gene (TaCRT1) Improves Salinity Tolerance in Tobacco.PloS one · 2015Article
- Callose homeostasis at plasmodesmata: molecular regulators and developmental relevance.Frontiers in plant science · 2014Article
- Loss of all three calreticulins, CRT1, CRT2 and CRT3, causes enhanced sensitivity to water stress in Arabidopsis.Plant cell reports · 2013Article
- Ectopic expression of a maize calreticulin mitigates calcium deficiency-like disorders in sCAX1-expressing tobacco and tomato.Plant molecular biology · 2012Article
- The function of calreticulin in plant immunity: new discoveries for an old protein.Plant signaling & behavior · 2012Article
- An ER-targeted calcium-binding peptide confers salt and drought tolerance mediated by CIPK6 in Arabidopsis.Planta · 2012Article
- Plant organellar calcium signalling: an emerging field.Journal of experimental botany · 2012Review
- Identification of genes required for nonhost resistance to Xanthomonas oryzae pv. oryzae reveals novel signaling components.PloS one · 2012Article
- Diverging functions among calreticulin isoforms in higher plants.Plant signaling & behavior · 2011Article
- Article
- Basal signaling regulates plant growth and development.Plant physiology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To investigate the endoplasmic reticulum (ER) Ca(2+) stores in plant cells, we generated tobacco (Nicotiana tabacum; NT1) suspension cells and Arabidopsis plants with altered levels of calreticulin (CRT), an ER-localized Ca(2+)-binding protein. NT1 cells and Arabidopsis plants were transformed with a maize (Zea mays) CRT gene in both sense and antisense orientations under the control of an Arabidopsis heat shock promoter. ER-enriched membrane fractions from NT1 cells were used to examine how altered expression of CRT affects Ca(2+) uptake and release. We found that a 2.5-fold increase in CRT led to a 2-fold increase in ATP-dependent (45)Ca(2+) accumulation in the ER-enriched fraction compared with heat-shocked wild-type controls. Furthermore, after treatment with the Ca(2+) ionophore ionomycin, ER microsomes from NT1 cells overproducing CRT showed a 2-fold increase in the amount of (45)Ca(2+) released, and a 2- to 3-fold increase in the amount of (45)Ca(2+) retained compared with wild type. These data indicate that altering the production of CRT affects the ER Ca(2+) pool. In addition, CRT transgenic Arabidopsis plants were used to determine if altered CRT levels had any physiological effects. We found that the level of CRT in heat shock-induced CRT transgenic plants correlated positively with the retention of chlorophyll when the plants were transferred from Ca(2+)-containing medium to Ca(2+)-depleted medium. Together these data are consistent with the hypothesis that increasing CRT in the ER increases the ER Ca(2+) stores and thereby enhances the survival of plants grown in low Ca(2+) medium.
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Registered trials
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