ArticleGenetics2001
Identification of a polymorphic mucin-like gene expressed in the midgut of the mosquito, Aedes aegypti, using an integrated bulked segregant and differential display analysis.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Deltamethrin Selection Drives Transcriptomic Changes in Detoxification, Immune, and Cuticle Genes inTropical medicine and infectious disease · 2025Article
- Genome-Wide Transcriptome Profiling Reveals Genes Associated with Meiotic Drive System ofInsects · 2019Article
- A transcriptomic survey of the impact of environmental stress on response to dengue virus in the mosquito, Aedes aegypti.PLoS neglected tropical diseases · 2018Article
- Evaluation of reference genes at different developmental stages for quantitative real-time PCR in Aedes aegypti.Scientific reports · 2017Article
- Ironing out the Details: Exploring the Role of Iron and Heme in Blood-Sucking Arthropods.Frontiers in physiology · 2017Review
- A database of circadian and diel rhythmic gene expression in the yellow fever mosquito Aedes aegypti.BMC genomics · 2014Article
- Stage-specific transcription during development of Aedes aegypti.BMC developmental biology · 2013Article
- Comparative analysis of midgut bacterial communities of Aedes aegypti mosquito strains varying in vector competence to dengue virus.Parasitology research · 2013Article
- RNAi knock-downs support roles for the mucin-like (AeIMUC1) gene and short-chain dehydrogenase/reductase (SDR) gene in Aedes aegypti susceptibility to Plasmodium gallinaceum.Medical and veterinary entomology · 2012Article
- Article
- Global cross-talk of genes of the mosquito Aedes aegypti in response to dengue virus infection.PLoS neglected tropical diseases · 2011Article
- Aedes aegypti vectorial capacity is determined by the infecting genotype of dengue virus.The American journal of tropical medicine and hygiene · 2006Article
- Monoclonal antibody MG96 completely blocks Plasmodium yoelii development in Anopheles stephensi.Infection and immunity · 2003Article
- A targeted approach to the identification of candidate genes determining susceptibility to Plasmodium gallinaceum in Aedes aegypti.Molecular genetics and genomics : MGG · 2003Article
Corrections and comments
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2 authors.
Funding
Abstract
The identification of putative differentially expressed genes within genome regions containing QTL determining susceptibility of the mosquito, Aedes aegypti, to the malarial parasite, Plasmodium gallinaceum, was investigated using an integrated, targeted approach based on bulked segregant and differential display analysis. A mosquito F2 population was obtained from pairwise matings between the parasite-susceptible RED strain and the resistant MOYO-R substrain. DNA from female carcasses was used to genotype individuals at RFLP markers of known chromosomal position around the major QTL (pgs 1). Midguts, dissected 48 hr after an infected blood meal, were used to prepare two RNA bulks, each representing one of the parental genotypes at the QTL interval. The RNA bulks were compared by differential display PCR. A mucin-like protein gene (AeIMUC1) was isolated and characterized. The gene maps within the pgs 1 QTL interval and is expressed in the adult female midgut. AeIMUC1 RNA abundance decreased with time after blood meal ingestion. No differential expression was observed between the two mosquito strains but three different alleles with inter- and intrastrain allelic polymorphisms including indels and SNPs were characterized. The AeIMUC1 gene chromosome location and allelic polymorphisms raise the possibility that the protein might be involved in parasite-mosquito interactions.
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