ArticleGenetics2001
Mutants of Chlamydomonas reinhardtii deficient in mitochondrial complex I: characterization of two mutations affecting the nd1 coding sequence.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 50 citations in OpenAlex.
- Review
- Antares I: a Modular Photobioreactor Suitable for Photosynthesis and Bioenergetics Research.Applied biochemistry and biotechnology · 2024Article
- Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Article
- Article
- Mitochondria Affect Photosynthetic Electron Transport and Photosensitivity in a Green Alga.Plant physiology · 2018Article
- Impaired Mitochondrial Transcription Termination Disrupts the Stromal Redox Poise inPlant physiology · 2017Article
- Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.PLoS genetics · 2012Article
- Characterization of an internal type-II NADH dehydrogenase from Chlamydomonas reinhardtii mitochondria.Current genetics · 2012Article
- Interplay between non-photochemical plastoquinone reduction and re-oxidation in pre-illuminated Chlamydomonas reinhardtii: a chlorophyll fluorescence study.Photosynthesis research · 2011Article
- Metabolic network reconstruction of Chlamydomonas offers insight into light-driven algal metabolism.Molecular systems biology · 2011Article
- A forward genetic screen identifies mutants deficient for mitochondrial complex I assembly in Chlamydomonas reinhardtii.Genetics · 2011Article
- Knock-down of the COX3 and COX17 gene expression of cytochrome c oxidase in the unicellular green alga Chlamydomonas reinhardtii.Plant molecular biology · 2010Article
- Eukaryotic complex I: functional diversity and experimental systems to unravel the assembly process.Molecular genetics and genomics : MGG · 2008Review
- ND3 and ND4L subunits of mitochondrial complex I, both nucleus encoded in Chlamydomonas reinhardtii, are required for activity and assembly of the enzyme.Eukaryotic cell · 2006Article
- High-efficiency biolistic transformation of Chlamydomonas mitochondria can be used to insert mutations in complex I genes.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Regulation of the alternative oxidase Aox1 gene in Chlamydomonas reinhardtii. Role of the nitrogen source on the expression of a reporter gene under the control of the Aox1 promoter.Plant physiology · 2003Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mitochondrial rotenone-sensitive NADH:ubiquinone oxidoreductase (complex I) comprises more than 30 subunits, the majority of which are encoded by the nucleus. In Chlamydomonas reinhardtii, only five components of complex I are coded for by mitochondrial genes. Three mutants deprived of complex I activity and displaying slow growth in the dark were isolated after mutagenic treatment with acriflavine. A genetical analysis demonstrated that two mutations (dum20 and dum25) affect the mitochondrial genome whereas the third mutation (dn26) is of nuclear origin. Recombinational analyses showed that dum20 and dum25 are closely linked on the genetic map of the mitochondrial genome and could affect the nd1 gene. A sequencing analysis confirmed this conclusion: dum20 is a deletion of one T at codon 243 of nd1; dum25 corresponds to a 6-bp deletion that eliminates two amino acids located in a very conserved hydrophilic segment of the protein.
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