ArticleGenetics2001
Eukaryotic beta-alanine synthases are functionally related but have a high degree of structural diversity.
Article in Genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Article
- Pantothenate and CoA biosynthesis in Apicomplexa and their promise as antiparasitic drug targets.PLoS pathogens · 2021Review
- Article
- Taxonomic Distribution of Cytochrome P450 Monooxygenases (CYPs) among the Budding Yeasts (Sub-PhylumMicroorganisms · 2019Article
- Prolonged antibiotic use induces intestinal injury in mice that is repaired after removing antibiotic pressure: implications for empiric antibiotic therapy.Metabolomics : Official journal of the Metabolomic Society · 2014Article
- Global expression analysis of the yeast Lachancea (Saccharomyces) kluyveri reveals new URC genes involved in pyrimidine catabolism.Eukaryotic cell · 2014Article
- Mutational and structural analysis of L-N-carbamoylase reveals new insights into a peptidase M20/M25/M40 family member.Journal of bacteriology · 2012Article
- Ergogenic effects of β-alanine and carnosine: proposed future research to quantify their efficacy.Nutrients · 2012Review
- The metabolism of histamine in the Drosophila optic lobe involves an ommatidial pathway: β-alanine recycles through the retina.The Journal of experimental biology · 2012Article
- A functional analysis of the pyrimidine catabolic pathway in Arabidopsis.The New phytologist · 2009Article
- Potential application of N-carbamoyl-beta-alanine amidohydrolase from Agrobacterium tumefaciens C58 for beta-amino acid production.Applied and environmental microbiology · 2009Article
- Crystallization and preliminary crystallographic studies of the recombinant L-N-carbamoylase from Geobacillus stearothermophilus CECT43.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Crystallization and preliminary X-ray data analysis of beta-alanine synthase from Drosophila melanogaster.Acta crystallographica. Section F, Structural biology and crystallization communications · 2007Article
- Purification, crystallization and X-ray diffraction analysis of dihydropyrimidinase from Dictyostelium discoideum.Acta crystallographica. Section F, Structural biology and crystallization communications · 2006Article
- Horizontal gene transfer promoted evolution of the ability to propagate under anaerobic conditions in yeasts.Molecular genetics and genomics : MGG · 2004Article
- Cell division defects of Schizosaccharomyces pombe liz1- mutants are caused by defects in pantothenate uptake.Eukaryotic cell · 2004Article
- Dihydropyrimidine amidohydrolases and dihydroorotases share the same origin and several enzymatic properties.Nucleic acids research · 2003Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
beta-Alanine synthase (EC 3.5.1.6), which catalyzes the final step of pyrimidine catabolism, has only been characterized in mammals. A Saccharomyces kluyveri pyd3 mutant that is unable to grow on N-carbamyl-beta-alanine as the sole nitrogen source and exhibits diminished beta-alanine synthase activity was used to clone analogous genes from different eukaryotes. Putative PYD3 sequences from the yeast S. kluyveri, the slime mold Dictyostelium discoideum, and the fruit fly Drosophila melanogaster complemented the pyd3 defect. When the S. kluyveri PYD3 gene was expressed in S. cerevisiae, which has no pyrimidine catabolic pathway, it enabled growth on N-carbamyl-beta-alanine as the sole nitrogen source. The D. discoideum and D. melanogaster PYD3 gene products are similar to mammalian beta-alanine synthases. In contrast, the S. kluyveri protein is quite different from these and more similar to bacterial N-carbamyl amidohydrolases. All three beta-alanine synthases are to some degree related to various aspartate transcarbamylases, which catalyze the second step of the de novo pyrimidine biosynthetic pathway. PYD3 expression in yeast seems to be inducible by dihydrouracil and N-carbamyl-beta-alanine, but not by uracil. This work establishes S. kluyveri as a model organism for studying pyrimidine degradation and beta-alanine production in eukaryotes.
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