ArticleAmerican journal of human genetics2001
Functional complementation of a genetic deficiency with human artificial chromosomes.
Article in American journal of human genetics, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 107 citations in OpenAlex.
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Analysis of complement system and its related factors in Alzheimer's disease.BMC neurology · 2023Article
- Episomes and Transposases-Utilities to Maintain Transgene Expression from Nonviral Vectors.Genes · 2022Review
- Precise Characterization and Tracking of Stably Inherited Artificial Minichromosomes Made by Telomere-Mediated Chromosome Truncation inFrontiers in plant science · 2021Article
- Gene expressing human artificial chromosome vectors: Advantages and challenges for gene therapy.Experimental cell research · 2020Review
- Human AlphoidCells · 2020Article
- Review
- A pathway from chromosome transfer to engineering resulting in human and mouse artificial chromosomes for a variety of applications to bio-medical challenges.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- De novo formed satellite DNA-based mammalian artificial chromosomes and their possible applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Genetic and epigenetic regulation of centromeres: a look at HAC formation.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Developing de novo human artificial chromosomes in embryonic stem cells using HSV-1 amplicon technology.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2015Review
- Comparative study of artificial chromosome centromeres in human and murine cells.European journal of human genetics : EJHG · 2013Article
- A new generation of human artificial chromosomes for functional genomics and gene therapy.Cellular and molecular life sciences : CMLS · 2013Review
- Organization of synthetic alphoid DNA array in human artificial chromosome (HAC) with a conditional centromere.ACS synthetic biology · 2012Article
- Human artificial chromosomes for gene delivery and the development of animal models.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Functional human artificial chromosomes are generated and stably maintained in human embryonic stem cells.Human molecular genetics · 2011Article
- Prospects for the use of artificial chromosomes and minichromosome-like episomes in gene therapy.Journal of biomedicine & biotechnology · 2010Article
- Manipulating transgenes using a chromosome vector.Nucleic acids research · 2009Article
- HAC stability in murine cells is influenced by nuclear localization and chromatin organization.BMC cell biology · 2009Article
- Simultaneous detection of FISH signals and bromo-deoxyuridine incorporation in fixed tissue cultured cells.PloS one · 2009Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 2 countries.
Funding
Abstract
We have shown functional complementation of a genetic deficiency in human cultured cells, using artificial chromosomes derived from cloned human genomic fragments. A 404-kb human-artificial-chromosome (HAC) vector, consisting of 220 kb of alphoid DNA from the centromere of chromosome 17, human telomeres, and the hypoxanthine guanine phosphoribosyltransferase (HPRT) genomic locus, was transferred to HPRT-deficient HT1080 fibrosarcoma cells. We generated several cell lines with low-copy-number, megabase-sized HACs containing a functional centromere and one or possibly several copies of the HPRT1 gene complementing the metabolic deficiency. The HACs consisted of alternating alphoid and nonalphoid DNA segments derived only from the input DNA (within the sensitivity limits of FISH detection), and the largest continuous alphoid segment was 158-250 kb. The study of both the structure and mitotic stability of these HACs offers insights into the mechanisms of centromere formation in synthetic chromosomes and will further the development of this human-gene-transfer technology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.