ArticleMolecular biology of the cell2001
Alanine-scanning mutagenesis of Aspergillus gamma-tubulin yields diverse and novel phenotypes.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 58 citations in OpenAlex.
- The tubulin database: Linking mutations, modifications, ligands and local interactions.PloS one · 2023Article
- Molecular insight into how γ-TuRC makes microtubules.Journal of cell science · 2021Review
- The γ-tubulin-specific inhibitor gatastatin reveals temporal requirements of microtubule nucleation during the cell cycle.Nature communications · 2015Article
- Spatial regulation of the spindle assembly checkpoint and anaphase-promoting complex in Aspergillus nidulans.Molecular microbiology · 2015Article
- NME7 is a functional component of the γ-tubulin ring complex.Molecular biology of the cell · 2014Article
- GTP regulates the microtubule nucleation activity of γ-tubulin.Nature cell biology · 2013Article
- γ-Tubulin plays a key role in inactivating APC/C(Cdh1) at the G(1)-S boundary.The Journal of cell biology · 2012Article
- The GCP3-interacting proteins GIP1 and GIP2 are required for γ-tubulin complex protein localization, spindle integrity, and chromosomal stability.The Plant cell · 2012Article
- The Polo-like kinase PLKA in Aspergillus nidulans is not essential but plays important roles during vegetative growth and development.Eukaryotic cell · 2012Article
- The functions of myosin II and myosin V homologs in tip growth and septation in Aspergillus nidulans.PloS one · 2012Article
- Gamma-tubulin regulates the anaphase-promoting complex/cyclosome during interphase.The Journal of cell biology · 2010Article
- In vivo analysis of the functions of gamma-tubulin-complex proteins.Journal of cell science · 2009Article
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.Molecular biology of the cell · 2008Article
- Role of microtubules in tip growth of fungi.Journal of plant research · 2007Article
- Gamma-tubulin is essential for microtubule organization and development in Arabidopsis.The Plant cell · 2006Article
- The Drosophila gamma-tubulin small complex subunit Dgrip84 is required for structural and functional integrity of the spindle apparatus.Molecular biology of the cell · 2006Article
- Mutational analyses reveal a novel function of the nucleotide-binding domain of gamma-tubulin in the regulation of basal body biogenesis.The Journal of cell biology · 2005Article
- Cytoplasmic dynein's mitotic spindle pole localization requires a functional anaphase-promoting complex, gamma-tubulin, and NUDF/LIS1 in Aspergillus nidulans.Molecular biology of the cell · 2005Article
- Overexpression of truncated gamma-tubulins disrupts mitotic aster formation in Xenopus oocyte extracts.The Biochemical journal · 2005Article
- Effects of {gamma}-tubulin complex proteins on microtubule nucleation and catastrophe in fission yeast.Molecular biology of the cell · 2005Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
We have created 41 clustered charged-to-alanine scanning mutations of the mipA, gamma-tubulin, gene of Aspergillus nidulans and have created strains carrying these mutations by two-step gene replacement and by a new procedure, heterokaryon gene replacement. Most mutant alleles confer a wild-type phenotype, but others are lethal or conditionally lethal. The conditionally lethal alleles exhibit a variety of phenotypes under restrictive conditions. Most have robust but highly abnormal mitotic spindles and some have abnormal cytoplasmic microtubule arrays. Two alleles appear to have reduced amounts of gamma-tubulin at the spindle pole bodies and nucleation of spindle microtubule assembly may be partially inhibited. One allele inhibits germ tube formation. The cold sensitivity of two alleles is strongly suppressed by the antimicrotubule agents benomyl and nocodazole and a third allele is essentially dependent on these compounds for growth. Together our data indicate that gamma-tubulin probably carries out functions essential to mitosis and organization of cytoplasmic microtubules in addition to its well-documented role in microtubule nucleation. We have also placed our mutations on a model of the structure of gamma-tubulin and these data give a good initial indication of the functionally important regions of the molecule.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.