ArticleMolecular biology of the cell2001
High PKC alpha and low E-cadherin expression contribute to high migratory activity of colon carcinoma cells.
Article in Molecular biology of the cell, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 113 citations in OpenAlex.
- Regulation of E-cadherin by mixed lineage kinase 3 (MLK3) mediates cell adhesion in ovarian cancer spheroids.Biochimica et biophysica acta. Molecular cell research · 2026Article
- Selective chemical and genetic inhibition of PKCβII strongly sensitizes colon cancer cells to anoikis.Molecular biology reports · 2025Article
- CXCR4/CXCL12 blockade therapy; a new horizon in TNBC therapy.Medical oncology (Northwood, London, England) · 2025Review
- Potential of Natural Products in the Treatment of Glioma: Focus on Molecular Mechanisms.Cell biochemistry and biophysics · 2024Review
- Spatiotemporal Regulation of FMNL2 by N-Terminal Myristoylation and C-Terminal Phosphorylation Drives Rapid Filopodia Formation.Biomolecules · 2023Article
- Aurora kinase A induces migration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells.BMB reports · 2022Article
- ATF4/CEMIP/PKCα promotes anoikis resistance by enhancing protective autophagy in prostate cancer cells.Cell death & disease · 2022Article
- The Pyrazolyl-Urea Gege3 Inhibits the Activity of ANXA1 in the Angiogenesis Induced by the Pancreatic Cancer Derived EVs.Biomolecules · 2021Article
- The complexities of PKCα signaling in cancer.Advances in biological regulation · 2021Review
- Neuroendocrine Factors and Head and Neck Squamous Cell Carcinoma: An Affair to Remember.Disease markers · 2018Review
- Inhibition of PKCα reduces the ability of migration of kidney cancer cells but has no impact on cell apoptosis.Experimental and therapeutic medicine · 2017Article
- Interacting post-muscarinic receptor signaling pathways potentiate matrix metalloproteinase-1 expression and invasion of human colon cancer cells.The Biochemical journal · 2017Article
- Low expression of PKCα and high expression of KRAS predict poor prognosis in patients with colorectal cancer.Oncology letters · 2016Article
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- Review
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- PKCα promotes the mesenchymal to amoeboid transition and increases cancer cell invasiveness.BMC cancer · 2015Article
- HER2 and uPAR cooperativity contribute to metastatic phenotype of HER2-positive breast cancer.Oncoscience · 2015Review
- Targeting tight junctions during epithelial to mesenchymal transition in human pancreatic cancer.World journal of gastroenterology · 2014Review
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Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The protein kinase C (PKC) is a family of serine/threonine kinases that are key regulatory enzymes involved in growth, differentiation, cytoskeletal reorganization, tumor promotion, and migration. We investigated the functional involvement of PKC isotypes and of E-cadherin in the regulation of the locomotion of six human colon-adenocarcinoma cell lines. The different levels of the PKC alpha and the E-cadherin expression have predictable implications in the spontaneous locomotory activity. With the use of PKC alpha--specific inhibitors (safingol, Go6976) as well as the PKC delta--specific inhibitor rottlerin, we showed that only PKC alpha plays a major role in the regulation of tumor cell migration. The results were verified by knocking out the translation of PKC isozymes with the use of an antisense oligonucleotide strategy. After stimulation with phorbol ester we observed a translocation and a colocalization of the activated PKC alpha at the plasma membrane to the surrounding extracellular matrix. Furthermore, we investigated the functional involvement of E-cadherin in the locomotion with the use of a blocking antibody. A high level of PKC alpha expression together with a low E-cadherin expression was strongly related to a high migratory activity of the colon carcinoma cells. This correlation was independent of the differentiation grade of the tumor cell lines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.