ArticleThe Journal of cell biology2001
Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.
Article in The Journal of cell biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 115 citations in OpenAlex.
- Actomyosin dynamics in detached cells: linking clutch model to cell migration and cytokinesis.BMC molecular and cell biology · 2025Article
- Role of myosin heavy chain 9 in gastrointestinal tumorigenesis: A comprehensive review.World journal of gastrointestinal oncology · 2025Review
- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Wound Repair of the Cell Membrane: Lessons fromCells · 2024Review
- Discovery and Quantitative Dissection of Cytokinesis Mechanisms Using Dictyostelium discoideum.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Dynamics of Actin Cytoskeleton and Their Signaling Pathways during Cellular Wound Repair.Cells · 2022Article
- Cell behaviors within a confined adhesive area fabricated using novel micropatterning methods.PloS one · 2022Article
- Article
- Time-varying mobility and turnover of actomyosin ring components during cytokinesis inMolecular biology of the cell · 2021Article
- The Ringleaders: Understanding the Apicomplexan Basal Complex Through Comparison to Established Contractile Ring Systems.Frontiers in cellular and infection microbiology · 2021Review
- CaCells · 2020Review
- Review
- Dynamin-Like Protein B ofCells · 2019Article
- Molecular Mechanism of Cytokinesis.Annual review of biochemistry · 2019Review
- Article
- Actin turnover ensures uniform tension distribution during cytokinetic actomyosin ring contraction.Molecular biology of the cell · 2019Article
- Manipulation of cell migration by laserporation-induced local wounding.Scientific reports · 2019Article
- Mechanisms of contractile ring tension production and constriction.Biophysical reviews · 2018Review
- A positive-feedback-based mechanism for constriction rate acceleration during cytokinesis ineLife · 2018Article
- A study of wound repair in Dictyostelium cells by using novel laserporation.Scientific reports · 2018Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myosin II is a major component of a contractile ring. To examine if myosin II turns over in contractile rings, fluorescence of GFP-myosin II expressed in Dictyostelium cells was bleached locally by laser illumination, and the recovery was monitored. The fluorescence recovered with a half time of 7.01 +/- 2.62 s. This recovery was not caused by lateral movement of myosin II from the nonbleached area, but by an exchange with endoplasmic myosin II. Similar experiments were performed in cells expressing GFP-3ALA myosin II, of which three phosphorylatable threonine residues were replaced with alanine residues. In this case, recovery was not detected within a comparable time range. These results indicate that myosin II in the contractile ring performs dynamic turnover via its heavy chain phosphorylation. Because GFP-3ALA myosin II did not show the recovery, it served as a useful marker of myosin II movement, which enabled us to demonstrate cortical flow of myosin II toward the equator for the first time. Thus, cortical flow accompanies the dynamic exchange of myosin II during the formation of contractile rings.
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