ArticleProceedings of the National Academy of Sciences of the United States of America2001
Sir3-dependent assembly of supramolecular chromatin structures in vitro.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 61 citations in OpenAlex.
- Unwrap RAP1's Mystery at Kinetoplastid Telomeres.Biomolecules · 2024Review
- Article
- Sir3 mediates long-range chromosome interactions in budding yeast.Genome research · 2021Article
- The Nuts and Bolts of Transcriptionally Silent Chromatin in Saccharomyces cerevisiae.Genetics · 2016Review
- Solution-state conformation and stoichiometry of yeast Sir3 heterochromatin fibres.Nature communications · 2014Article
- The role of the nucleosome acidic patch in modulating higher order chromatin structure.Journal of the Royal Society, Interface · 2013Review
- Dimerization of Sir3 via its C-terminal winged helix domain is essential for yeast heterochromatin formation.The EMBO journal · 2013Article
- Structure and function in the budding yeast nucleus.Genetics · 2012Review
- Regulating repression: roles for the sir4 N-terminus in linker DNA protection and stabilization of epigenetic states.PLoS genetics · 2012Article
- Structural basis for the role of the Sir3 AAA+ domain in silencing: interaction with Sir4 and unmethylated histone H3K79.Genes & development · 2011Article
- Modeling of chromosomal epigenetic silencing processes.Transcription · 2011Article
- Clustering heterochromatin: Sir3 promotes telomere clustering independently of silencing in yeast.The Journal of cell biology · 2011Article
- Reconstitution of heterochromatin-dependent transcriptional gene silencing.Molecular cell · 2009Article
- Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling.Cell · 2009Article
- Mutational analysis of the Sir3 BAH domain reveals multiple points of interaction with nucleosomes.Molecular and cellular biology · 2009Article
- Phylogenetic conservation and homology modeling help reveal a novel domain within the budding yeast heterochromatin protein Sir1.Molecular and cellular biology · 2009Article
- Synthetic lethal screens identify gene silencing processes in yeast and implicate the acetylated amino terminus of Sir3 in recognition of the nucleosome core.Molecular and cellular biology · 2008Article
- The silent information regulator 3 protein, SIR3p, binds to chromatin fibers and assembles a hypercondensed chromatin architecture in the presence of salt.Molecular and cellular biology · 2008Article
- Long-range communication between the silencers of HMR.Molecular and cellular biology · 2008Article
- Conversion of a replication origin to a silencer through a pathway shared by a Forkhead transcription factor and an S phase cyclin.Molecular biology of the cell · 2008Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Baculovirus-expressed recombinant Sir3p (rSir3p) has been purified to near homogeneity, and its binding to naked DNA, mononucleosomes, and nucleosomal arrays has been characterized in vitro. At stoichiometric levels rSir3p interacts with intact nucleosomal arrays, mononucleosomes, and naked DNA, as evidenced by formation of supershifted species on native agarose gels. Proteolytic removal of the core histone tail domains inhibits but does not completely abolish rSir3p binding to nucleosomal arrays. The linker DNA in the supershifted complexes remains freely accessible to restriction endonuclease digestion, suggesting that both the tail domains and nucleosomal DNA contribute to rSir3p--chromatin interactions. Together these data indicate that rSir3p cross-links individual nucleosomal arrays into supramolecular assemblies whose physical properties transcend those of typical 10-nm and 30-nm fibers. Based on these data we hypothesize that Sir3p functions, at least in part, by mediating reorganization of the canonical chromatin fiber into functionally specialized higher order chromosomal domains.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.