ArticleProceedings of the National Academy of Sciences of the United States of America2001
Histone deacetylase-dependent transcriptional repression by pRB in yeast occurs independently of interaction through the LXCXE binding cleft.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Targeting epigenetics for cancer therapy.Archives of pharmacal research · 2019Review
- tRNA modifications regulate translation during cellular stress.FEBS letters · 2014Review
- A system of RNA modifications and biased codon use controls cellular stress response at the level of translation.Chemical research in toxicology · 2014Article
- Msi1-Like (MSIL) Proteins in Fungi.Mycobiology · 2013Article
- Pleiotropic roles of the Msi1-like protein Msl1 in Cryptococcus neoformans.Eukaryotic cell · 2012Article
- An overlapping kinase and phosphatase docking site regulates activity of the retinoblastoma protein.Nature structural & molecular biology · 2010Article
- A-type nuclear lamins act as transcriptional repressors when targeted to promoters.Experimental cell research · 2009Article
- Article
- High mobility group protein HMGA1 inhibits retinoblastoma protein-mediated cellular G0 arrest.Cancer science · 2007Article
- Mutual interdependence of MSI1 (CAC3) and YAK1 in Saccharomyces cerevisiae.Journal of molecular biology · 2007Article
- MTB, the murine homolog of condensin II subunit CAP-G2, represses transcription and promotes erythroid cell differentiation.Leukemia · 2006Article
- p55, the Drosophila ortholog of RbAp46/RbAp48, is required for the repression of dE2F2/RBF-regulated genes.Molecular and cellular biology · 2004Article
- Inhibition of retinoblastoma protein degradation by interaction with the serpin plasminogen activator inhibitor 2 via a novel consensus motif.Molecular and cellular biology · 2003Article
- Saccharomyces cerevisiae chromatin-assembly factors that act during DNA replication function in the maintenance of genome stability.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- The Human Adenovirus Type 5 E4orf6/E1B55K E3 Ubiquitin Ligase Complex Can Mimic E1A Effects on E2F.mSphereArticle
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have developed a yeast model system to address transcriptional repression by the retinoblastoma protein (pRB). When fused to the DNA-binding domain of Gal4p (DB-pRB), pRB can repress transcription of reporter genes containing Gal4p binding sites; the histone deacetylase activity encoded by yeast RPD3 is required for DB-pRB repression. Mutation of the LXCXE binding cleft in pRB, a region reported to be required for histone deacetylase recruitment, does not interfere with pRB-mediated repression. From these findings based on yeast experiments, we surmise that the small pocket region of pRB must contain an additional domain that confers histone deacetylase-dependent transcriptional repression. This hypothesis was verified by experiments examining pRB-dependent histone deacetylase association in mammalian cells. In addition to RPD3, repression by pRB in yeast requires MSI1, an ortholog of RbAp48, but not SIN3 or SAP30. By comparing the genetic requirements of DB-pRB repression in yeast to those of other DB-repressor fusions, we can suggest a mechanism by which pRB recruits histone deacetylase activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.