ArticleProceedings of the National Academy of Sciences of the United States of America2001
A two-hybrid system for transactivator bait proteins.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 59 citations in OpenAlex.
- Yeast Heterologous Expression Systems for the Study of Plant Membrane Proteins.International journal of molecular sciences · 2023Review
- TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation.Communications biology · 2023Article
- Genetically Encoded Whole Cell Biosensor for Drug Discovery of HIF-1 Interaction Inhibitors.ACS synthetic biology · 2022Article
- Identifying and Validating MYC:Protein Interactors in Pursuit of Novel Anti-MYC Therapies.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Review
- Review
- Mutations in the ArsA ATPase that restore interaction with the ArsD metallochaperone.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2014Article
- DELLAs function as coactivators of GAI-ASSOCIATED FACTOR1 in regulation of gibberellin homeostasis and signaling in Arabidopsis.The Plant cell · 2014Article
- Drosophila heparan sulfate 6-O-endosulfatase Sulf1 facilitates wingless (Wg) protein degradation.The Journal of biological chemistry · 2013Article
- Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiology and molecular biology reviews : MMBR · 2012Review
- Genetic mapping of the interface between the ArsD metallochaperone and the ArsA ATPase.Molecular microbiology · 2011Article
- The Sos-recruitment system as a tool to analyze cellular localization of plant proteins: membrane localization of Arabidopsis thaliana PEPINO/PASTICCINO2.Molecular genetics and genomics : MGG · 2010Article
- C-TAK1 interacts with microphthalmia-associated transcription factor, Mitf, but not the related family member Tfe3.Biochemical and biophysical research communications · 2010Article
- The 19S proteasomal lid subunit POH1 enhances the transcriptional activation by Mitf in osteoclasts.Journal of cellular biochemistry · 2010Article
- TAF1 differentially enhances androgen receptor transcriptional activity via its N-terminal kinase and ubiquitin-activating and -conjugating domains.Molecular endocrinology (Baltimore, Md.) · 2010Article
- Yeast two-hybrid, a powerful tool for systems biology.International journal of molecular sciences · 2009Review
- BAR the door: cancer suppression by amphiphysin-like genes.Biochimica et biophysica acta · 2009Review
- Isolation and identification of L-dopa decarboxylase as a protein that binds to and enhances transcriptional activity of the androgen receptor using the repressed transactivator yeast two-hybrid system.The Biochemical journal · 2003Article
- Transcriptional regulation: a genomic overview.The arabidopsis book · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe a two-hybrid strategy for detection of interactions with transactivator proteins. This repressed transactivator (RTA) system employs the N-terminal repression domain of the yeast general repressor TUP1. TUP1-GAL80 fusion proteins, when coexpressed with GAL4, are shown to inhibit transcription of GAL4-dependent reporter genes. This effect requires the C-terminal 30 residues of GAL4, which are required for interaction with GAL80 in vitro. Furthermore, repression of GAL transcription by TUP1-GAL80 requires SRB10, demonstrating that the TUP1 repression domain, in the context of a two-hybrid interaction, functions by the same mechanism as endogenous TUP1. Using this strategy, we demonstrate interactions between the mammalian basic helix-loop-helix proteins MyoD and E12, and between c-Myc and Bin-1. We have also identified interacting clones from a TUP1-cDNA fusion expression library by using GAL4-VP16 as a bait fusion. These results demonstrate that RTA is generally applicable for identifying and characterizing interactions with transactivator proteins in vivo.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.