ArticleInfection and immunity2001
Identification of Listeria monocytogenes in vivo-induced genes by fluorescence-activated cell sorting.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 30 citations in OpenAlex.
- Using fluorescence flow cytometry data for single-cell gene expression analysis in bacteria.PloS one · 2020Article
- Applications of flow cytometry to characterize bacterial physiological responses.BioMed research international · 2014Review
- A differential fluorescence-based genetic screen identifies Listeria monocytogenes determinants required for intracellular replication.Journal of bacteriology · 2013Article
- Structure of fumarate hydratase from Rickettsia prowazekii, the agent of typhus and suspected relative of the mitochondria.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Single-cell techniques using chromosomally tagged fluorescent bacteria to study Listeria monocytogenes infection processes.Applied and environmental microbiology · 2010Article
- Identification of an essential gene of Listeria monocytogenes involved in teichoic acid biogenesis.Journal of bacteriology · 2006Article
- Intracellular gene expression profile of Listeria monocytogenes.Infection and immunity · 2006Article
- Identification of genes induced in Listeria monocytogenes during growth and attachment to cut cabbage, using differential display.Applied and environmental microbiology · 2005Article
- Large-scale quantitative analysis of sources of variation in the actin polymerization-based movement of Listeria monocytogenes.Biophysical journal · 2005Article
- Unraveling the secret lives of bacteria: use of in vivo expression technology and differential fluorescence induction promoter traps as tools for exploring niche-specific gene expression.Microbiology and molecular biology reviews : MMBR · 2005Review
- Single-copy green fluorescent protein gene fusions allow accurate measurement of Salmonella gene expression in vitro and during infection of mammalian cells.Applied and environmental microbiology · 2003Article
- Nontypeable Haemophilus influenzae gene expression induced in vivo in a chinchilla model of otitis media.Infection and immunity · 2003Article
- Identification of chromosomal Shigella flexneri genes induced by the eukaryotic intracellular environment.Infection and immunity · 2002Article
- Differential fluorescence induction analysis of Streptococcus pneumoniae identifies genes involved in pathogenesis.Infection and immunity · 2002Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Listeria monocytogenes is a gram-positive, intracellular, food-borne pathogen capable of causing severe infections in immunocompromised or pregnant individuals, as well as numerous animal species. Genetic analysis of Listeria pathogenesis has identified several genes which are crucial for virulence. The transcription of most of these genes has been shown to be induced upon entry of Listeria into the host cell. To identify additional genes that are induced in vivo and may be required for L. monocytogenes pathogenesis, a fluorescence-activated cell-sorting technique was initiated. Random fragments of the L. monocytogenes chromosome were cloned into a plasmid carrying a promoterless green fluorescent protein (GFP) gene, and the plasmids were transformed into the L. monocytogenes actA mutant DP-L1942. Fluorescence-activated cell sorting (FACS) was used to isolate L. monocytogenes clones that exhibited increased GFP expression within macrophage-like J774 cells but had relatively low levels of GFP expression when the bacteria were extracellular. Using this strategy, several genes were identified, including actA, that exhibited such an expression profile. In-frame deletions of two of these genes, one encoding the putative L. monocytogenes uracil DNA glycosylase (ung) and one encoding a protein with homology to the Bacillus subtilis YhdP hemolysin-like protein, were constructed and introduced into the chromosome of wild-type L. monocytogenes 10403s. The L. monocytogenes 10403s ung deletion mutant was not attenuated for virulence in mice, while the yhdP mutant exhibited a three- to sevenfold reduction in virulence.
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