ArticleInfection and immunity2001
Nickel-responsive induction of urease expression in Helicobacter pylori is mediated at the transcriptional level.
Article in Infection and immunity, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 124 citations in OpenAlex.
- CirculatingFrontiers in cellular and infection microbiology · 2026Article
- Fur-regulated urease contributes to the environmental adaptation ofMicrobiology spectrum · 2025Article
- Metal homeostasis in pathogenic Epsilonproteobacteria: mechanisms of acquisition, efflux, and regulation.Metallomics : integrated biometal science · 2021Review
- Schiff bases and their metal complexes as urease inhibitors - A brief review.Journal of advanced research · 2018Review
- A review on the development of urease inhibitors as antimicrobial agents against pathogenic bacteria.Journal of advanced research · 2018Review
- Measurement of Internal pH in Helicobacter pylori by Using Green Fluorescent Protein Fluorimetry.Journal of bacteriology · 2018Article
- Article
- Nickel Ligation of the N-Terminal Amine of HypA Is Required for Urease Maturation in Helicobacter pylori.Biochemistry · 2017Article
- Elucidation of the Metabolic Network of Helicobacter pylori J99 and Malaysian Clinical Strains by Phenotype Microarray.Helicobacter · 2017Article
- Coiled coil rich proteins (Ccrp) influence molecular pathogenicity of Helicobacter pylori.PloS one · 2015Article
- The Regulation of the AdcR Regulon in Streptococcus pneumoniae Depends Both on Zn(2+)- and Ni(2+)-Availability.Frontiers in cellular and infection microbiology · 2015Article
- Localization of FtsZ in Helicobacter pylori and consequences for cell division.Journal of bacteriology · 2013Article
- Inhibition of Helicobacter pylori urease activity in vivo by the synthetic nickel binding protein Hpn.European journal of microbiology & immunology · 2013Article
- Helicobacter pylori 5'ureB-sRNA, a cis-encoded antisense small RNA, negatively regulates ureAB expression by transcription termination.Journal of bacteriology · 2013Article
- RND multidrug efflux pumps: what are they good for?Frontiers in microbiology · 2013Article
- A convenient and robust in vivo reporter system to monitor gene expression in the human pathogen Helicobacter pylori.Applied and environmental microbiology · 2012Article
- A cis-encoded antisense small RNA regulated by the HP0165-HP0166 two-component system controls expression of ureB in Helicobacter pylori.Journal of bacteriology · 2011Article
- Mua (HP0868) is a nickel-binding protein that modulates urease activity in Helicobacter pylori.mBio · 2011Article
- Helicobacter pylori urease activity is influenced by ferric uptake regulator.Yonsei medical journal · 2010Article
- Molecular and biochemical characterization of urease and survival of Yersinia enterocolitica biovar 1A in acidic pH in vitro.BMC microbiology · 2009Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nickel-containing enzyme urease is an essential colonization factor of the gastric pathogen Helicobacter pylori, as it allows the bacterium to survive the acidic conditions in the gastric mucosa. Although urease can represents up to 10% of the total protein content of H. pylori, expression of urease genes is thought to be constitutive. Here it is demonstrated that H. pylori regulates the expression and activity of its urease enzyme as a function of the availability of the cofactor nickel. Supplementation of brucella growth medium with 1 or 100 microM NiCl(2) resulted in up to 3.5-fold-increased expression of the urease subunit proteins UreA and UreB and up to 12-fold-increased urease enzyme activity. The induction was specific for nickel, since the addition of cadmium, cobalt, copper, iron, manganese, or zinc did not affect the expression of urease. Both Northern hybridization studies and a transcriptional ureA::lacZ fusion demonstrated that the observed nickel-responsive regulation of urease is mediated at the transcriptional level. Mutation of the HP1027 gene, encoding the ferric uptake regulator (Fur), did not affect the expression of urease in unsupplemented medium but reduced the nickel induction of urease expression to only twofold. This indicates that Fur is involved in the modulation of urease expression in response to nickel. These data demonstrate nickel-responsive regulation of H. pylori urease, a phenomenon likely to be of importance during the colonization and persistence of H. pylori in the gastric mucosa.
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