ArticleAnalytical biochemistry2001
Fluorescence lifetime characterization of novel low-pH probes.
Article in Analytical biochemistry, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 139 citations in OpenAlex.
- Rab14 restricts pathogens by promoting V-ATPase lysosomal delivery to drive lysosomal acidification.Nature communications · 2026Article
- Autofluorescence lifetime of gelatin-methacrylate hydrogels is sensitive to changes in crosslinking and post-gelation pH.Frontiers in bioengineering and biotechnology · 2026Article
- Targeting pH Inversion in Prostate Cancer Cells: A Role for Systems of Molecules of Vegetal Origin.International journal of molecular sciences · 2025Article
- Quantitative profiling pH heterogeneity of acidic endolysosomal compartments using fluorescence lifetime imaging microscopy.Molecular biology of the cell · 2025Article
- Exploring lysosomal biology: current approaches and methods.Biophysics reports · 2024Article
- Organelle proteomic profiling reveals lysosomal heterogeneity in association with longevity.eLife · 2024Article
- Understanding How Cationic Polymers' Properties Inform Toxic or Immunogenic Responses via Parametric Analysis.Macromolecules · 2023Article
- Carbon Dots Embedded Hybrid Microgel with Phenylboronic Acid as Monomer for Fluorescent Glucose Sensing and Glucose-Triggered Insulin Release at Physiological pH.Nanomaterials (Basel, Switzerland) · 2022Article
- A lysosomal regulatory circuit essential for the development and function of microglia.Science advances · 2022Article
- Loss of KEAP1 Causes an Accumulation of Nondegradative Organelles.Antioxidants (Basel, Switzerland) · 2022Article
- Ppt1-deficiency dysregulates lysosomal CaJournal of inherited metabolic disease · 2022Article
- Intracellular pH Control by Membrane Transport in Mammalian Cells. Insights Into the Selective Advantages of Functional Redundancy.Frontiers in molecular biosciences · 2022Review
- Electrocatalytic Reaction Driven Flow: Role of pH in Flow Reversal.The journal of physical chemistry. C, Nanomaterials and interfaces · 2021Article
- A zinc transporter, transmembrane protein 163, is critical for the biogenesis of platelet dense granules.Blood · 2021Article
- Methods Used to Investigate theFrontiers in cellular and infection microbiology · 2021Review
- Different Sensitivity of Macrophages to Phospholipidosis Induction by Amphiphilic Cationic Drugs.International journal of molecular sciences · 2020Article
- α-Synuclein Overexpression Induces Lysosomal Dysfunction and Autophagy Impairment in Human Neuroblastoma SH-SY5Y.Neurochemical research · 2020Article
- Gold nanoclusters elicit homeostatic perturbations in glioblastoma cells and adaptive changes of lysosomes.Theranostics · 2020Article
- SNX11 Identified as an Essential Host Factor for SFTS Virus Infection by CRISPR Knockout Screening.Virologica Sinica · 2019Article
- Fluorescent Probes Based on π-Conjugation Modulation between Hemicyanine and Coumarin Moieties for Ratiometric Detection of pH Changes in Live Cells with Visible and Near-infrared Channels.Sensors and actuators. B, Chemical · 2018Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The structures and functions of the cellular acidic compartments are strongly dependent on the pH gradients across vesicular membranes. Measurement and imaging of the vesicular pH require fluorophores with appropriate pK(a) values. In this report, we characterized the pH-dependent lifetime responses of a family of acidotropic probes, LysoSensors, to evaluate their usefulness to low-pH lifetime imaging. LysoSensors are cell-permeable weak bases that selectively accumulate in acidic vesicles after being protonated. They have higher quantum yields at lower pH ranges to allow visualization of the lysosomes. For LysoSensors DND-167, DND-189, and DND-153, raising the buffer pH increased the quenching effects of their basic side chains and substantially reduced their steady-state fluorescence and lifetimes. The apparent pK(a) values determined from their lifetime responses were shifted to near neutral values because of the dominant intensity contribution from their protonated species. One unique property of LysoSensor DND-189 is its nonmonotonic lifetime responses of the maxima occurring between pH 4 and 5. LysoSensor DND-192 did not show significant lifetime changes over a wide pH range. LysoSensor DND-160, which was the only excitation and emission ratiometric probe, showed significant pH-dependent lifetime changes as well as its spectral shifts. Its apparent pK(a) values determined from the lifetime responses were comparable to the lysosomal pH because of its bright basic form. Because of the pH-dependent absorption spectra, the apparent pK(a) values could be manipulated between 3 and 5 by changing the excitation and/or emission wavelengths. These results indicate that LysoSensor DND-160 is a promising probe for lifetime imaging to determine lysosomal pH.
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