ArticleJournal of bacteriology2001
Novel topology of BfpE, a cytoplasmic membrane protein required for type IV fimbrial biogenesis in enteropathogenic Escherichia coli.
Article in Journal of bacteriology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 50 citations in OpenAlex.
- In-Feed vs. In-Water Chlortetracycline Administration on the Fecal Prevalence of Virulence Genes and Pathotypes ofMicroorganisms · 2025Article
- Type IV pili ofEcoSal Plus · 2024Review
- Crystal Structure of the Minor Pilin CofB, the Initiator of CFA/III Pilus Assembly in Enterotoxigenic Escherichia coli.The Journal of biological chemistry · 2015Article
- Characterization and localization of the Campylobacter jejuni transformation system proteins CtsE, CtsP, and CtsX.Journal of bacteriology · 2015Article
- The Inner Membrane Protein PilG Interacts with DNA and the Secretin PilQ in Transformation.PloS one · 2015Article
- Editorial.FEMS microbiology reviews · 2015Article
- Exceptionally widespread nanomachines composed of type IV pilins: the prokaryotic Swiss Army knives.FEMS microbiology reviews · 2015Review
- Structural and functional characterization of Pseudomonas aeruginosa global regulator AmpR.Journal of bacteriology · 2014Article
- Type IV pili in Gram-positive bacteria.Microbiology and molecular biology reviews : MMBR · 2013Review
- BfpL is essential for type IV bundle-forming pilus biogenesis and interacts with the periplasmic face of BfpC.Microbiology (Reading, England) · 2012Article
- Structure of an essential type IV pilus biogenesis protein provides insights into pilus and type II secretion systems.Journal of molecular biology · 2012Article
- Pseudomonas aeruginosa β-lactamase induction requires two permeases, AmpG and AmpP.BMC microbiology · 2010Article
- The Cpx envelope stress response both facilitates and inhibits elaboration of the enteropathogenic Escherichia coli bundle-forming pilus.Molecular microbiology · 2010Article
- Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins.Molecular microbiology · 2009Article
- The three-dimensional structure of the cytoplasmic domains of EpsF from the type 2 secretion system of Vibrio cholerae.Journal of structural biology · 2009Article
- Characterization of CetA and CetB, a bipartite energy taxis system in Campylobacter jejuni.Molecular microbiology · 2008Article
- Type IV pili: paradoxes in form and function.Current opinion in structural biology · 2008Review
- Purification and three-dimensional electron microscopy structure of the Neisseria meningitidis type IV pilus biogenesis protein PilG.Journal of bacteriology · 2007Article
- The ATPase activity of BfpD is greatly enhanced by zinc and allosteric interactions with other Bfp proteins.The Journal of biological chemistry · 2005Article
- The Cpx envelope stress response affects expression of the type IV bundle-forming pili of enteropathogenic Escherichia coli.Journal of bacteriology · 2005Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Enteropathogenic Escherichia coli (EPEC) produces the bundle-forming pilus (BFP), a type IV fimbria that has been implicated in virulence, autoaggregation, and localized adherence to epithelial cells. The bfpE gene is one of a cluster of bfp genes previously shown to encode functions that direct BFP biosynthesis. Here, we show that an EPEC strain carrying a nonpolar mutation in bfpE fails to autoaggregate, adhere to HEp-2 cells, or form BFP, thereby demonstrating that BfpE is required for BFP biogenesis. BfpE is a cytoplasmic membrane protein of the GspF family. To determine the membrane topology of BfpE, we fused bfpE derivatives containing 3' truncations and/or internal deletions to alkaline phosphatase and/or beta-galactosidase reporter genes, whose products are active only when localized to the periplasm or cytoplasm, respectively. In addition, we constructed BfpE sandwich fusions using a dual alkaline phosphatase/beta-galactosidase reporter cassette and analyzed BfpE deletion derivatives by sucrose density flotation gradient fractionation. The data from these analyses support a topology in which BfpE contains four hydrophobic transmembrane (TM) segments, a large cytoplasmic segment at its N terminus, and a large periplasmic segment near its C terminus. This topology is dramatically different from that of OutF, another member of the GspF family, which has three TM segments and is predominantly cytoplasmic. These findings provide a structural basis for predicting protein-protein interactions required for assembly of the BFP biogenesis machinery.
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