ArticleThe Biochemical journal2001
Sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12) from Desulfonispora thiosulfatigenes: purification, properties and primary sequence.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 45 citations in OpenAlex.
- A class of metallohydrolases expands bile salt hydrolase activity in the gut.bioRxiv : the preprint server for biology · 2026Article
- Oxygenolytic sulfoquinovose degradation by an iron-dependent alkanesulfonate dioxygenase.iScience · 2023Article
- Ecophysiology and interactions of a taurine-respiring bacterium in the mouse gut.Nature communications · 2023Article
- Sulfoquinovose is a widespread organosulfur substrate for Roseobacter clade bacteria in the ocean.The ISME journal · 2023Article
- Two radical-dependent mechanisms for anaerobic degradation of the globally abundant organosulfur compound dihydroxypropanesulfonate.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- A Pathway for Isethionate Dissimilation in Bacillus krulwichiae.Applied and environmental microbiology · 2019Article
- Radical-mediated C-S bond cleavage in C2 sulfonate degradation by anaerobic bacteria.Nature communications · 2019Article
- A glycyl radical enzyme enables hydrogen sulfide production by the human intestinal bacteriumProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Function and Evolution of the Sox Multienzyme Complex in the Marine Gammaproteobacterium Congregibacter litoralis.ISRN microbiology · 2014Article
- Paracoccus denitrificans PD1222 utilizes hypotaurine via transamination followed by spontaneous desulfination to yield acetaldehyde and, finally, acetate for growth.Journal of bacteriology · 2013Article
- Gene clusters involved in isethionate degradation by terrestrial and marine bacteria.Applied and environmental microbiology · 2010Article
- Bifurcated degradative pathway of 3-sulfolactate in Roseovarius nubinhibens ISM via sulfoacetaldehyde acetyltransferase and (S)-cysteate sulfolyase.Journal of bacteriology · 2009Article
- L-cysteate sulpho-lyase, a widespread pyridoxal 5'-phosphate-coupled desulphonative enzyme purified from Silicibacter pomeroyi DSS-3(T).The Biochemical journal · 2006Article
- Sulphoacetaldehyde acetyltransferase yields acetyl phosphate: purification from Alcaligenes defragrans and gene clusters in taurine degradation.The Biochemical journal · 2003Article
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4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The strictly anaerobic bacterium Desulfonispora thiosulfatigenes ferments taurine via sulphoacetaldehyde, which is hydrolysed to acetate and sulphite by sulphoacetaldehyde sulpho-lyase (EC 4.4.1.12). The lyase was expressed at high levels and a two-step, 4.5-fold purification yielded an apparently homogeneous soluble protein, which was presumably a homodimer in its native form; the molecular mass of the subunit was about 61 kDa (by SDS/PAGE). The mass was determined to be 63.8 kDa by matrix-assisted laser-desorption ionization-time-of-flight (MALDI-TOF) MS. The purified enzyme converted 1 mol of sulphoacetaldehyde to 1 mol each of sulphite and acetate, but no requirement for thiamine pyrophosphate (TPP) was detected. The N-terminal and two internal amino acid sequences were determined, which allowed us to generate PCR primers. The gene was amplified and sequenced. The DNA sequence had no significant homologue in the databases searched, whereas the derived amino acid sequence indicated an oxo-acid lyase, revealed a TPP-binding site and gave a derived molecular mass of 63.8 kDa.
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