ArticleThe Biochemical journal2001
Green fluorescent protein (GFP) tagged to the cytoplasmic tail of alphaIIb or beta3 allows the expression of a fully functional integrin alphaIIb(beta3): effect of beta3GFP on alphaIIb(beta3) ligand binding.
Article in The Biochemical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 29 citations in OpenAlex.
- Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis.Methods in molecular biology (Clifton, N.J.) · 2023Article
- A functionally neutral single chain antibody to measure beta-1 integrin uptake and recycling.Traffic (Copenhagen, Denmark) · 2020Article
- Coupling integrin dynamics to cellular adhesion behaviors.Biology open · 2018Article
- Optogenetic interrogation of integrin αVβ3 function in endothelial cells.Journal of cell science · 2017Article
- Novel ecto-tagged integrins reveal their trafficking in live cells.Nature communications · 2017Article
- Membrane tension controls adhesion positioning at the leading edge of cells.The Journal of cell biology · 2017Article
- Integrin-dependent force transmission to the extracellular matrix by α-actinin triggers adhesion maturation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Integrins β1 and β3 exhibit distinct dynamic nanoscale organizations inside focal adhesions.Nature cell biology · 2012Article
- Force generated by actomyosin contraction builds bridges between adhesive contacts.The EMBO journal · 2010Article
- The novel S527F mutation in the integrin beta3 chain induces a high affinity alphaIIbbeta3 receptor by hindering adoption of the bent conformation.The Journal of biological chemistry · 2009Article
- Profiling distinct mechanisms of tumour invasion for drug discovery: imaging adhesion, signalling and matrix turnover.Clinical & experimental metastasis · 2009Review
- Spatial separation and bidirectional trafficking of proteins using a multi-functional reporter.BMC cell biology · 2008Article
- Disrupting integrin transmembrane domain heterodimerization increases ligand binding affinity, not valency or clustering.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- The primacy of affinity over clustering in regulation of adhesiveness of the integrin {alpha}L{beta}2.The Journal of cell biology · 2004Article
- Marching at the front and dragging behind: differential alphaVbeta3-integrin turnover regulates focal adhesion behavior.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using green fluorescent protein (GFP) as an autofluorescent tag, we report the first successful visualization of a beta3 integrin in a living cell. GFP fused in frame to the cytoplasmic tail of either alphaIIb or beta3 allowed normal expression, heterodimerization, processing and surface exposure of alphaIIbGFPbeta3 and alphaIIb(beta3)GFP receptors in Chinese hamster ovary (CHO) cells. Direct microscopic observation of the autofluorescent cells in suspension following antibody-induced alphaIIb(beta3) capping revealed an intense autofluorescent cap corresponding to unlabelled immunoclustered GFP-tagged alphaIIb(beta3). GFP-tagged alphaIIbbeta3 receptors mediated fibrinogen-dependent cell adhesion, were readily detectable in focal adhesions of unstained living cells and triggered p125(FAK) tyrosine phosphorylation similar to wild-type alphaIIb(beta3) (where FAK corresponds to focal adhesion kinase). However, GFP tagged to beta3, but not to alphaIIb, induced spontaneous CHO cell aggregation in the presence of soluble fibrinogen, as well as binding of the fibrinogen mimetic monoclonal antibody PAC1 in the absence of alphaIIb(beta3) receptor activation. Time-lapse imaging of living transfectants revealed a characteristic redistribution of GFP-tagged alphaIIb(beta3) during the early stages of cell attachment and spreading, starting with alphaIIb(beta3) clustering at the rim of the cell contact area, that gradually overlapped with the boundary of the attached cell, and, with the onset of cell spreading, to a reorganization of alphaIIb(beta3) in focal adhesions. Taken together, our results demonstrate that (1) fusion of GFP to the cytoplasmic tail of either alphaIIb or beta3 integrin subunits allows normal cell surface expression of a functional receptor, and (2) structural modification of the beta3 integrin cytoplasmic tail, rather than the alphaIIb subunit, plays a major role in alphaIIb(beta3) affinity modulation. With the successful direct visualization of functional alphaIIb(beta3) receptors in living cells, the generation of autofluorescent integrins in transgenic animals will become possible, allowing new approaches to study the dynamics of integrin functions.
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