ArticleProceedings of the National Academy of Sciences of the United States of America2001
An antisense approach to phenotype-based gene cloning in Tetrahymena.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Inferring gene-pathway associations from consolidated transcriptome datasets: an interactive gene network explorer forNAR genomics and bioinformatics · 2025Article
- Review
- Nested genes CDA12 and CDA13 encode proteins associated with membrane trafficking in the ciliate Tetrahymena thermophila.Eukaryotic cell · 2009Article
- Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway.Molecular and cellular biology · 2006Article
- The Tetrahymena thermophila phagosome proteome.Eukaryotic cell · 2006Article
- Distinct paths to stop codon reassignment by the variant-code organisms Tetrahymena and Euplotes.Molecular and cellular biology · 2006Article
- Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin.PLoS genetics · 2005Article
- Genetic, genomic, and functional analysis of the granule lattice proteins in Tetrahymena secretory granules.Molecular biology of the cell · 2005Article
- Responses of the ciliates Tetrahymena and Paramecium to external ATP and GTP.Purinergic signalling · 2005Article
- Core formation and the acquisition of fusion competence are linked during secretory granule maturation in Tetrahymena.Traffic (Copenhagen, Denmark) · 2005Article
- New class of cargo protein in Tetrahymena thermophila dense core secretory granules.Eukaryotic cell · 2002Article
- A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Analysis of a mutant exhibiting conditional sorting to dense core secretory granules in Tetrahymena thermophila.Genetics · 2001Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We report a pioneering approach using Tetrahymena thermophila that permits rapid identification of genes based on their null or hypomorphic phenotypes. This technique involves cell transformation with a library of plasmids that encode 26S ribosomal subunits containing short insertions. The insertions correspond to antisense sequences for a large number of genes. The majority of cells each acquires a single antisense sequence, which silences a single genomic locus. Because the insertion site within the ribosomal sequence is known, the silenced gene is easily amplified. We demonstrate that this approach can be used to identify genes required for dense core granule exocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.