ArticleMolecular and cellular biology2001
DNA replication forks pause at silent origins near the HML locus in budding yeast.
Article in Molecular and cellular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 38 citations in OpenAlex.
- Loss of G1-phase CDK-inhibition biases instability between genomic regions by unevenly reducing activity among replication origins.iScience · 2025Article
- The Ubiquitin Ligase TRAIP: Double-Edged Sword at the Replisome.Trends in cell biology · 2021Review
- tRNA Genes Affect Chromosome Structure and Function via Local Effects.Molecular and cellular biology · 2019Article
- Donor Preference Meets Heterochromatin: Moonlighting Activities of a Recombinational Enhancer in Saccharomyces cerevisiae.Genetics · 2016Article
- Chromosome Duplication in Saccharomyces cerevisiae.Genetics · 2016Review
- Rad51-Rad52 mediated maintenance of centromeric chromatin in Candida albicans.PLoS genetics · 2014Article
- Long-range heterochromatin association is mediated by silencing and double-strand DNA break repair proteins.The Journal of cell biology · 2013Article
- Replication fork stalling by bulky DNA damage: localization at active origins and checkpoint modulation.Nucleic acids research · 2011Article
- Replication termination at eukaryotic chromosomes is mediated by Top2 and occurs at genomic loci containing pausing elements.Molecular cell · 2010Article
- Systematic identification of fragile sites via genome-wide location analysis of gamma-H2AX.Nature structural & molecular biology · 2010Article
- Orchestration of the S-phase and DNA damage checkpoint pathways by replication forks from early origins.The Journal of cell biology · 2008Article
- Replication fork barriers: pausing for a break or stalling for time?EMBO reports · 2007Review
- Replication fork stalling at natural impediments.Microbiology and molecular biology reviews : MMBR · 2007Review
- Genome-wide replication profiles of S-phase checkpoint mutants reveal fragile sites in yeast.The EMBO journal · 2006Article
- Replication fork progression is impaired by transcription in hyperrecombinant yeast cells lacking a functional THO complex.Molecular and cellular biology · 2006Article
- A mutation in Dbf4 motif M impairs interactions with DNA replication factors and confers increased resistance to genotoxic agents.Molecular and cellular biology · 2005Article
- Molecular anatomy and regulation of a stable replisome at a paused eukaryotic DNA replication fork.Genes & development · 2005Article
- Diminished S-phase cyclin-dependent kinase function elicits vital Rad53-dependent checkpoint responses in Saccharomyces cerevisiae.Molecular and cellular biology · 2004Article
- The yeast Sgs1 helicase is differentially required for genomic and ribosomal DNA replication.The EMBO journal · 2003Article
- ORC and the intra-S-phase checkpoint: a threshold regulates Rad53p activation in S phase.Genes & development · 2002Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Chromosomal replicators in budding yeast contain an autonomously replicating sequence (ARS) that functions in a plasmid, but certain ARSs are silent as replication origins in their natural chromosomal context. In chromosome III, the HML ARS cluster (ARS302-ARS303-ARS320) and ARS301 flank the transcriptionally silent mating-type locus HML, and all of these ARSs are silent as replication origins. ARS301 and ARS302 function in transcriptional silencing mediated by the origin recognition complex (ORC) and a heterochromatin structure, while the functions of ARS303 and ARS320 are not known. In this work, we discovered replication fork pause sites at the HML ARS cluster and ARS301 by analyzing DNA replication intermediates from the chromosome via two-dimensional gel electrophoresis. The replication fork pause at the HML ARS cluster was independent of cis- and trans-acting mutations that abrogate transcriptional silencing at HML. Deletion of the HML ARS cluster led to loss of the pause site. Insertion of a single, heterologous ARS (ARS305) in place of the HML ARS cluster reconstituted the pause site, as did multiple copies of DNA elements (A and B1) that bind ORC. The orc2-1 mutation, known to alter replication timing at origins, did not detectably affect the pause but activated the silent origin at the HML ARS cluster in a minority of cells. Delaying the time of fork arrival at HML led to the elimination of the pause sites at the HML ARS cluster and at the copy of ARS305 inserted in place of the cluster. Loss of the pause sites was accompanied by activation of the silent origins in the majority of cells. Thus, replication fork movement near HML pauses at a silent origin which is competent for replication initiation but kept silent through Orc2p, a component of the replication initiator. Possible functions for replication fork pause sites in checkpoints, S-phase regulation, mating-type switching, and transcriptionally silent heterochromatin are discussed.
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