ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Stages of synapse development defined by dependence on F-actin.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 112 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
112 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- A PDZ-RapGEF promotes synaptic development in Caenorhabditis elegans through a Rap/Rac signaling pathway.Development (Cambridge, England) · 2025Article
- Regulation of synapse density by Pumilio RNA-binding proteins.Cell reports · 2024Article
- Neuronal maturation and axon regeneration: unfixing circuitry to enable repair.Nature reviews. Neuroscience · 2024Review
- Synapses without tension fail to fire in an in vitro network of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Presynapses contain distinct actin nanostructures.The Journal of cell biology · 2023Article
- Novel regenerative drug, SPG302 promotes functional recovery of diaphragm muscle activity after cervical spinal cord injury.The Journal of physiology · 2023Article
- Axonal plasticity in response to active forces generated through magnetic nano-pulling.Cell reports · 2023Article
- How does Nogo receptor influence demyelination and remyelination in the context of multiple sclerosis?Frontiers in cellular neuroscience · 2023Review
- An Update on the Critical Role of α-Synuclein in Parkinson's Disease and Other Synucleinopathies: from Tissue to Cellular and Molecular Levels.Molecular neurobiology · 2022Review
- The role of altered protein acetylation in neurodegenerative disease.Frontiers in aging neuroscience · 2022Review
- PXF-1 promotes synapse development at the neuromuscular junction inFrontiers in molecular neuroscience · 2022Article
- Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling.BMC biology · 2021Article
- GPR18 drives FAAH inhibition-induced neuroprotection against HIV-1 Tat-induced neurodegeneration.Experimental neurology · 2021Article
- Profilin2 regulates actin rod assembly in neuronal cells.Scientific reports · 2021Article
- PLPP/CIN-mediated NF2-serine 10 dephosphorylation regulates F-actin stability and Mdm2 degradation in an activity-dependent manner.Cell death & disease · 2021Article
- A kinesin adapter directly mediates dendritic mRNA localization during neural development in mice.The Journal of biological chemistry · 2020Article
- Linking Alpha-Synuclein to the Actin Cytoskeleton: Consequences to Neuronal Function.Frontiers in cell and developmental biology · 2020Review
- Intrinsic and extrinsic mechanisms of synapse formation and specificity in C. elegans.Cellular and molecular life sciences : CMLS · 2019Review
- Article
52 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
2 authors.
Funding
Abstract
It has been widely speculated that actin plays a central role in CNS synapse assembly, but such a requirement for actin filaments (F-actin) has not yet been demonstrated experimentally. We used hippocampal neurons grown in culture and the actin depolymerizing agent, latrunculin A, to examine directly the relationship between F-actin and synapse formation and maturation. During the first week in culture, actin depolymerization results in a near complete loss of synapses defined by synaptophysin-labeled vesicle clusters, synaptic vesicle recycling, and ultrastructure. Over the second week in culture, F-actin becomes increasingly stable, but actin depolymerization no longer disrupts basic synaptic structure. There is, however, a reduction in the number and size of synaptophysin-labeled clusters and in the size of vesicle clusters undergoing FM4-64 recycling, suggesting that synaptic vesicle anchoring remains partially dependent on F-actin. By 18 d in culture, synaptophysin clusters and synaptic vesicle recycling are largely resistant to F-actin depolymerization. The decrease in synapse dependence on F-actin correlates well with the acquisition and retention of presynaptic scaffolding proteins such as Bassoon and postsynaptic scaffolding proteins such as those of the postsynaptic density-95 family. Increased activity stabilizes F-actin and its associated proteins at synaptic sites, suggesting a correlation between active synapses, actin stability, and synapse stability. Our findings demonstrate that F-actin is essential for the development and maintenance of young synapses. Because F-actin is also highly regulatable, we propose that F-actin may be a principal target for stabilizing or destabilizing signals that ultimately result in synapse maintenance or elimination.
Indexed as
Identifiers
11438592PMC6762826What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.