ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2001
Dynamics of glycine receptor insertion in the neuronal plasma membrane.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The trial behind it
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Who cites it
24 citing papers in PubMed, 72 citations in OpenAlex.
- Inhibitory Receptor Diffusion Dynamics.Frontiers in molecular neuroscience · 2019Article
- Linking Nanoscale Dynamics of AMPA Receptor Organization to Plasticity of Excitatory Synapses and Learning.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Review
- Impaired Glycine Receptor Trafficking in Neurological Diseases.Frontiers in molecular neuroscience · 2018Review
- An aggregation-removal model for the formation and size determination of post-synaptic scaffold domains.PLoS computational biology · 2017Article
- Transport along the dendritic endoplasmic reticulum mediates the trafficking of GABAB receptors.Journal of cell science · 2014Article
- GABAA receptor membrane insertion rates are specified by their subunit composition.Molecular and cellular neurosciences · 2013Article
- Gephyrin, the enigmatic organizer at GABAergic synapses.Frontiers in cellular neuroscience · 2012Article
- Heat shock cognate protein 70 regulates gephyrin clustering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Gephyrin oligomerization controls GlyR mobility and synaptic clustering.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Cellular transport and membrane dynamics of the glycine receptor.Frontiers in molecular neuroscience · 2009Article
- Molecular architecture of glycinergic synapses.Histochemistry and cell biology · 2008Review
- Distinct subcellular distribution of delta-opioid receptor fused with various tags in PC12 cells.Neurochemical research · 2008Article
- Review
- Mechanisms of homomeric alpha1 glycine receptor endocytosis.Biochemistry · 2007Article
- Multiple association states between glycine receptors and gephyrin identified by SPT analysis.Biophysical journal · 2007Article
- Organelles and trafficking machinery for postsynaptic plasticity.Annual review of neuroscience · 2006Review
- Assembly-dependent surface targeting of the heterodimeric GABAB Receptor is controlled by COPI but not 14-3-3.Molecular biology of the cell · 2005Article
- Structural basis of dynamic glycine receptor clustering by gephyrin.The EMBO journal · 2004Article
- Nerve-independent formation of a topologically complex postsynaptic apparatus.The Journal of cell biology · 2004Article
- Intracellular association of glycine receptor with gephyrin increases its plasma membrane accumulation rate.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The exocytosis site of newly synthesized glycine receptor was defined by means of a morphological assay to characterize its export from the trans-Golgi Network to the plasma membrane. This was achieved by expressing in transfected neurons an alpha1 subunit bearing an N-terminal tag selectively cleavable from outside the cell by thrombin. This was combined with a transient temperature-induced block of exocytic transport that creates a synchronized exocytic wave. Immunofluorescence microscopy analysis of the cell surface appearance of newly synthesized receptor revealed that exocytosis mainly occurred at nonsynaptic sites in the cell body and the initial portion of dendrites. At the time of cell surface insertion, the receptors existed as discrete clusters. Quantitative analysis showed that glycine receptor clusters are stable in size and subsequently appeared in more distal dendritic regions. This localization resulted from diffusion in the plasma membrane and not from exocytosis of transport vesicles directed to dendrites. Kinetic analysis established a direct substrate-product relationship between pools of somatic and dendritic receptors. This indicated that clusters represent intermediates between newly synthesized and synaptic receptors. These results support a diffusion-retention model for the formation of receptor-enriched postsynaptic domains and not that of a vectorial intracellular targeting to synapses.
Indexed as
Identifiers
11438579PMC6762839W1934415635What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.