ArticleTransgenic research2001
Post-transcriptional gene silencing in plum pox virus resistant transgenic European plum containing the plum pox potyvirus coat protein gene.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 158 citations in OpenAlex.
- Envisaging the role of plant Small RNAs: A comprehensive review on antiviral defense mechanisms.Molecular biology reports · 2026Review
- CRISPR/Cas as a Genome-Editing Technique in Fruit Tree Breeding.International journal of molecular sciences · 2023Review
- Trans-grafting plum pox virus resistance from transgenic plum rootstocks to apricot scions.Frontiers in plant science · 2023Article
- A versatileFrontiers in plant science · 2022Article
- Optimized RNA-Silencing Strategies forPlants (Basel, Switzerland) · 2021Article
- Robust Response toGenes · 2021Article
- Long-Term Efficacy and Safety of RNAi-Mediated Virus Resistance in 'HoneySweet' Plum.Frontiers in plant science · 2021Article
- Defining the 'HoneySweet' insertion event utilizing NextGen sequencing and a de novo genome assembly of plum (Prunus domestica).Horticulture research · 2021Article
- Engineering plant virus resistance: from RNA silencing to genome editing strategies.Plant biotechnology journal · 2020Review
- Innovative RNAi Strategies and Tactics to Tackle Plum Pox Virus (PPV) Genome inPlants (Basel, Switzerland) · 2019Article
- RNAi-Mediated Resistance Against Viruses in Perennial Fruit Plants.Plants (Basel, Switzerland) · 2019Review
- Multilocation comparison of fruit composition for 'HoneySweet', an RNAi based plum pox virus resistant plum.PloS one · 2019Article
- Article
- Current achievements and future directions in genetic engineering of European plum (Prunus domestica L.).Transgenic research · 2018Review
- An efficient viral vector for functional genomic studies of Prunus fruit trees and its induced resistance to Plum pox virus via silencing of a host factor gene.Plant biotechnology journal · 2017Article
- New Biotechnological Tools for the Genetic Improvement of Major Woody Fruit Species.Frontiers in plant science · 2017Review
- Small RNA Based Genetic Engineering for Plant Viral Resistance: Application in Crop Protection.Frontiers in microbiology · 2017Review
- Use of Posttranscription Gene Silencing in Squash to Induce Resistance against the Egyptian Isolate of the Squash Leaf Curl Virus.International journal of genomics · 2016Article
- Review
- Biotechnological strategies and tools for Plum pox virus resistance: trans-, intra-, cis-genesis, and beyond.Frontiers in plant science · 2015Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgenic plums containing the plum pox potyvirus coat protein (PPV-CP) gene were inoculated with PPV. Infection was monitored by evaluating symptoms, ELISA, and IC-RT-PCR. Transgenic clone C5 was highly resistant to PPV during four years of testing and displayed characteristics typical of post-transcriptional gene silencing (PTGS), including a high level of transgene transcription in the nucleus, low levels of transgene mRNA in the cytoplasm, a complex multicopy transgene insertion with aberrant copies, and methylation of the silenced PPV-CP transgene. The PPV-CP transgene was also methylated in seedlings of C5 and these seedlings were resistant to PPV. Our results show, for the first time, that PTGS functions as a mechanism for virus resistance in a woody perennial species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.