ArticleTransgenic research2001
Expression of recombinant human granulocyte macrophage-colony stimulating factor (hGM-CSF) in mouse urine.
Article in Transgenic research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 30 citations in OpenAlex.
- A Novel Methanol-Free Platform for Extracellular Expression of rhGM-CSF in Pichia pastoris.Molecular biotechnology · 2019Article
- Expression systems and species used for transgenic animal bioreactors.BioMed research international · 2013Article
- Expression of hGM-CSF in silk glands of transgenic silkworms using gene targeting vector.Transgenic research · 2012Article
- Dynamic control of oligosaccharide modification in the mammary gland: linking recombinant human erythropoietin functional analysis of transgenic mouse milk-derived hEPO.Transgenic research · 2006Article
- Goat uromodulin promoter directs kidney-specific expression of GFP gene in transgenic mice.BMC biotechnology · 2005Article
- Generation and phenotypic analysis of a transgenic line of rabbits secreting active recombinant human erythropoietin in the milk.Transgenic research · 2004Article
- Renal tubule-specific expression and urinary secretion of human growth hormone: a kidney-based transgenic bioreactor growth.Transgenic research · 2003Article
- The use of the uromodulin promoter to target production of recombinant proteins into urine of transgenic animals.Transgenic research · 2002Article
- Uromodulin promoter directs high-level expression of biologically active human alpha1-antitrypsin into mouse urine.The Biochemical journal · 2002Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have generated transgenic mice expressing human granulocyte macrophage-colony stimulating factor (hGM-CSF) in urine. In particular, the expression plasmid DNA containing mouse uroplakin II promoter was used to direct uroepithelium-specific transcription of transgene. In this study, hGM-CSF transcript was detected only in bladder uroepithelium as determined by northern blot analysis. Furthermore, hGM-CSF protein was detected in the suprabasal layer of the uroepithelium and ureter by immunohistochemistry. The hGM-CSF was secreted into urine at high level (up to 180 ng/ml), and enhanced proliferation of hGM-CSF-dependent human acute monocyte leukemic cells, suggesting that transgenic urine-derived hGM-CSF was bioactive. This is the first case of demonstrating biological activity of a cytokine produced in the urine of a transgenic animal. Our results demonstrate that bladder can be used as a bioreactor to produce biologically important substances. In addition, it suggests a potential application of bladder expression system to livestock for high-yield production of pharmaceuticals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.