ArticlePlant molecular biology2001
A novel orfB-related gene of carrot mitochondrial genomes that is associated with homeotic cytoplasmic male sterility (CMS).
Article in Plant molecular biology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- From genomic conflict to agricultural innovation: nuclear surveillance through spatiotemporal restriction underlies cytoplasmic male sterility.Science China. Life sciences · 2026Article
- Ubiquitin-mediated degradation restricts spatiotemporal accumulation of the cytoplasmic male sterility protein WA352 to anthers in rice.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Differences in homologous and heterologous nucleocytoplasmic interactions of cytoplasmic male sterility lines in Gossypium barbadense.Scientific reports · 2025Article
- Past and future of cytoplasmic male sterility and heterosis breeding in crop plants.Plant cell reports · 2025Review
- Protoplast Technology and Somatic Hybridisation in the Family Apiaceae.Plants (Basel, Switzerland) · 2023Review
- The Mitochondrial Genome Assembly of Fennel (International journal of molecular sciences · 2020Article
- Differentially Expressed Genes between Carrot Petaloid Cytoplasmic Male Sterile and Maintainer during Floral Development.Scientific reports · 2019Article
- Comprehensive transcriptome-based characterization of differentially expressed genes involved in microsporogenesis of radish CMS line and its maintainer.Functional & integrative genomics · 2016Article
- Basic helix-loop-helix transcription factor BcbHLHpol functions as a positive regulator of pollen development in non-heading Chinese cabbage.Functional & integrative genomics · 2014Article
- Mitochondrial atp9 genes from petaloid male-sterile and male-fertile carrots differ in their status of heteroplasmy, recombination involvement, post-transcriptional processing as well as accumulation of RNA and protein product.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2014Article
- Instability in mitochondrial membranes in Polima cytoplasmic male sterility of Brassica rapa ssp. chinensis.Functional & integrative genomics · 2014Article
- Proteome analysis of the wild and YX-1 male sterile mutant anthers of wolfberry (Lycium barbarum L.).PloS one · 2012Article
- Analysis of genetic diversity in cytoplasmic male sterility, and association of mitochondrial genes with petaloid-type cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee).Molecular biology reports · 2010Article
- Gametophytically alloplasmic CMS line of rice (Oryza sativa L.) with variant orfH79 haplotype corresponds to specific fertility restorer.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2008Article
- Homeotic-like modification of stamens to petals is associated with aberrant mitochondrial gene expression in cytoplasmic male sterile Ogura Brassica juncea.Journal of genetics · 2006Article
- Petaloid-type cms in carrot is not associated with expression of atp8 (orfB).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2006Article
- A 60 kDa COX1 protein in mitochondria of carrot irrespective of the presence of C-terminal extensions in the cox1 reading frames.Molecular genetics and genomics : MGG · 2006Article
- Alloplasmic male sterility in Brassica napus (CMS 'Tournefortii-Stiewe') is associated with a special gene arrangement around a novel atp9 gene.Molecular genetics and genomics : MGG · 2003Article
- ORFB is a subunit of F1F(O)-ATP synthase: insight into the basis of cytoplasmic male sterility in sunflower.EMBO reports · 2003Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three types of orfB-related genes, orfB-F1, orfB-F2 and orfB-CMS, were found in carrot mitochondrial genomes. OrfB-F1 has a structure similar to the reported orfB gene in sunflower, and orfB-F2 is a novel chimeric gene with about 200 bp of unknown sequence at the 3' end of the orfB-related sequence. All fertile plants examined contained either orfB-F1 or orfB-F2. OrfB-CMS is also a novel chimeric orfB-related gene with an additional 170 bp of unknown sequence at the 3' end. Fifteen carrot lines including petaloid CMS were examined by PCR analysis and all petaloid CMS plants were found to contain the orfB-CMS gene. The orfB-F2 and orfB-CMS genes coexist in three lines, and these lines exhibit a CMS phenotype, suggesting that the CMS phenotype is associated with orfB-CMS and is independent of the presence of orfB-F2. Interestingly, differences in predicted amino acid sequence between orfB-CMS and orfB-F2 were very limited and restricted to the carboxy-terminal region of the protein. The orfB-related genes were transcribed as expected from their DNA structures, but orfB-CMS protein accumulated only in floral organs of the CMS lines. Four RNA editing sites were common to orfB-CMS and orfB-F2, and C-to-U editing occurred in both floral and leaf organs for orfB-CMS. These results strongly suggest that the orfB-CMS gene is closely associated with the petaloid phenotype and its expression is not regulated by RNA editing, but by post-transcriptional events.
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