ArticleGenome research2001
High-throughput plasmid purification for capillary sequencing.
Article in Genome research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 56 citations in OpenAlex.
- Diagnostic Value of Next-Generation Sequencing in Periprosthetic Joint Infection: A Systematic Review.Orthopaedic surgery · 2022Pooled it
- Phylogenetics and genetic variation ofJournal of nematology · 2021Article
- First report of molecular characterization and phylogeny ofJournal of nematology · 2020Article
- Paths of Heritable Mitochondrial DNA Mutation and Heteroplasmy in Reference and gas-1 Strains of Caenorhabditis elegans.Frontiers in genetics · 2016Article
- High rate of large deletions in Caenorhabditis briggsae mitochondrial genome mutation processes.Genome biology and evolution · 2009Article
- Molecular evolution in Panagrolaimus nematodes: origins of parthenogenesis, hermaphroditism and the Antarctic species P. davidi.BMC evolutionary biology · 2009Article
- Intron presence-absence polymorphisms in Daphnia.Molecular biology and evolution · 2008Article
- Ameiotic recombination in asexual lineages of Daphnia.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- The relative roles of three DNA repair pathways in preventing Caenorhabditis elegans mutation accumulation.Genetics · 2006Article
- Facile recovery of individual high-molecular-weight, low-copy-number natural plasmids for genomic sequencing.Applied and environmental microbiology · 2006Article
- Purification and preconcentration of genomic DNA from whole cell lysates using photoactivated polycarbonate (PPC) microfluidic chips.Nucleic acids research · 2006Article
- High-throughput isolation of ultra-pure plasmid DNA by a robotic system.BMC biotechnology · 2006Article
- Detection of diverse new Francisella-like bacteria in environmental samples.Applied and environmental microbiology · 2005Article
- Mutation rates, spectra and hotspots in mismatch repair-deficient Caenorhabditis elegans.Genetics · 2005Article
- High-throughput sequencing: a failure mode analysis.BMC genomics · 2005Article
- Abundance, distribution, and mutation rates of homopolymeric nucleotide runs in the genome of Caenorhabditis elegans.Journal of molecular evolution · 2004Article
- Transcript analysis of genes encoding a family 61 endoglucanase and a putative membrane-anchored family 9 glycosyl hydrolase from Phanerochaete chrysosporium.Applied and environmental microbiology · 2002Article
- Glass bead purification of plasmid template DNA for high throughput sequencing of mammalian genomes.Nucleic acids research · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The need for expeditious and inexpensive methods for high-throughput DNA sequencing has been highlighted by the accelerated pace of genome DNA sequencing over the past year. At the Joint Genome Institute, the throughput in terms of high-quality bases per day has increased over 20-fold during the past 18 mo, reaching an average of 18.3 million bases per day. To support this unprecedented scaleup, we developed an inexpensive automated method for the isolation and purification of double-stranded plasmid DNA clones for sequencing that is tailored to meet the more stringent needs of the newer capillary electrophoresis DNA sequencing machines. The protocol is based on the magnetic bead method of solid phase reversible immobilization that has been automated by using a CRS-based robotic system. The method described here has enabled us to meet our increases in production while reducing labor and materials costs significantly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.