ArticleNature biotechnology2001
A rapid, generally applicable method to engineer zinc fingers illustrated by targeting the HIV-1 promoter.
Article in Nature biotechnology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- DNA recognition, cleavage, and toxic metal ion interaction of an artificial zinc finger protein inside E. coli cells.Protein science : a publication of the Protein Society · 2026Article
- Production of Homogeneous, Functional Zinc-Finger Arrays in High Yield With Two Chromatographic Steps.Bio-protocol · 2025Article
- Article
- Application of Deep Sequencing in Phage Display.Methods in molecular biology (Clifton, N.J.) · 2024Article
- A universal deep-learning model for zinc finger design enables transcription factor reprogramming.Nature biotechnology · 2023Article
- TReSR: A PCR-compatible DNA sequence design method for engineering proteins containing tandem repeats.PloS one · 2023Article
- Genome Editing and Diabetic Cardiomyopathy.Advances in experimental medicine and biology · 2023Article
- Comparison of the Feasibility, Efficiency, and Safety of Genome Editing Technologies.International journal of molecular sciences · 2021Review
- CRISPR/Cas-Dependent and Nuclease-FreeHuman gene therapy · 2021Review
- Protein Delivery of Cell-Penetrating Zinc-Finger Activators Stimulates Latent HIV-1-Infected Cells.Molecular therapy. Methods & clinical development · 2020Article
- Engineering altered protein-DNA recognition specificity.Nucleic acids research · 2018Review
- Intracellular directed evolution of proteins from combinatorial libraries based on conditional phage replication.Nature protocols · 2017Article
- Targeted mutagenesis: A sniper-like diversity generator in microbial engineering.Synthetic and systems biotechnology · 2017Review
- Binding Site Configurations Probe the Structure and Dynamics of the Zinc Finger of NEMO (NF-κB Essential Modulator).Biochemistry · 2017Article
- An ensemble micro neural network approach for elucidating interactions between zinc finger proteins and their target DNA.BMC genomics · 2016Article
- Exploiting the recognition code for elucidating the mechanism of zinc finger protein-DNA interactions.BMC genomics · 2016Article
- Zebrafish Genome Engineering Using the CRISPR-Cas9 System.Trends in genetics : TIG · 2016Review
- Genome-Editing Technologies: Principles and Applications.Cold Spring Harbor perspectives in biology · 2016Review
- Near-complete elimination of mutant mtDNA by iterative or dynamic dose-controlled treatment with mtZFNs.Nucleic acids research · 2016Article
- Deimmunization for gene therapy: host matching of synthetic zinc finger constructs enables long-term mutant Huntingtin repression in mice.Molecular neurodegeneration · 2016Article
22 more citing papers are in PubMed but not listed here.
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3 authors.
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Abstract
DNA-binding domains with predetermined sequence specificity are engineered by selection of zinc finger modules using phage display, allowing the construction of customized transcription factors. Despite remarkable progress in this field, the available protein-engineering methods are deficient in many respects, thus hampering the applicability of the technique. Here we present a rapid and convenient method that can be used to design zinc finger proteins against a variety of DNA-binding sites. This is based on a pair of pre-made zinc finger phage-display libraries, which are used in parallel to select two DNA-binding domains each of which recognizes given 5 base pair sequences, and whose products are recombined to produce a single protein that recognizes a composite (9 base pair) site of predefined sequence. Engineering using this system can be completed in less than two weeks and yields proteins that bind sequence-specifically to DNA with Kd values in the nanomolar range. To illustrate the technique, we have selected seven different proteins to bind various regions of the human immunodeficiency virus 1 (HIV-1) promoter.
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