ArticleNucleic acids research2001
Significantly higher activity of a cytoplasmic hammerhead ribozyme than a corresponding nuclear counterpart: engineered tRNAs with an extended 3' end can be exported efficiently and specifically to the cytoplasm in mammalian cells.
Article in Nucleic acids research, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 40 citations in OpenAlex.
- Coexpressed RIG-I agonist enhances humoral immune response to influenza virus DNA vaccine.Journal of virology · 2011Article
- Allele-specific RNAi mitigates phenotypic progression in a transgenic model of Alzheimer's disease.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome.Virology journal · 2009Article
- tRNA-linked molecular beacons for imaging mRNAs in the cytoplasm of living cells.Nucleic acids research · 2005Article
- Targeting mortalin using conventional and RNA-helicase-coupled hammerhead ribozymes.EMBO reports · 2003Article
- The stable 2.0-kilobase intron of the herpes simplex virus type 1 latency-associated transcript does not function as an antisense repressor of ICP0 in nonneuronal cells.Journal of virology · 2003Article
- Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells.Nucleic acids research · 2003Article
- Functional repair of a mutant chloride channel using a trans-splicing ribozyme.The Journal of clinical investigation · 2002Article
- Plant 7SL RNA and tRNA(Tyr) genes with inserted antisense sequences are efficiently expressed in an in vitro transcription system from Nicotiana tabacum cells.Plant molecular biology · 2002Article
- Endogenous expression of a high-affinity pseudoknot RNA aptamer suppresses replication of HIV-1.Nucleic acids research · 2002Article
- A functional gene discovery in the Fas-mediated pathway to apoptosis by analysis of transiently expressed randomized hybrid-ribozyme libraries.Nucleic acids research · 2002Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hammerhead ribozymes were expressed under the control of similar tRNA promoters, localizing transcripts either in the cytoplasm or the nucleus. The tRNA(Val)-driven ribozyme (tRNA-Rz; tRNA with extra sequences at the 3' end) that has been used in our ribozyme studies was exported efficiently into the cytoplasm and ribozyme activity was detected only in the cytoplasmic fraction. Both ends of the transported tRNA-Rz were characterized comprehensively and the results confirmed that tRNA-Rz had unprocessed 5' and 3' ends. Furthermore, it was also demonstrated that the activity of the exported ribozyme was significantly higher than that of the ribozyme which remained in the nucleus. We suggest that it is possible to engineer tRNA-Rz, which can be exported to the cytoplasm based on an understanding of secondary structures, and then tRNA-driven ribozymes may be co-localized with their target mRNAs in the cytoplasm of mammalian cells.
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