ArticleThe Journal of physiology2001
Release of ATP by a human retinal pigment epithelial cell line: potential for autocrine stimulation through subretinal space.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 108 citations in OpenAlex.
- Review of the clinical electrooculogram - Part 1: Mechanism of the Light-Rise.Documenta ophthalmologica. Advances in ophthalmology · 2026Review
- The short wavelength electro-oculogram (SW-EOG) in best disease: preliminary results.Documenta ophthalmologica. Advances in ophthalmology · 2026Article
- The Healthy and Diseased Retina Seen through Neuron-Glia Interactions.International journal of molecular sciences · 2024Review
- Endogenous and Exogenous Regulation of Redox Homeostasis in Retinal Pigment Epithelium Cells: An Updated Antioxidant Perspective.International journal of molecular sciences · 2023Review
- Article
- Hyposmotic stress causes ATP release in a discrete zone within the outer cortex of rat lens.Molecular vision · 2022Article
- Is white the right light for the clinical electrooculogram?Documenta ophthalmologica. Advances in ophthalmology · 2021Article
- Purinergic signaling in the retina: From development to disease.Brain research bulletin · 2019Review
- Polarized retinal pigment epithelium generates electrical signals that diminish with age and regulate retinal pathology.Journal of cellular and molecular medicine · 2018Article
- Modulation of three key innate immune pathways for the most common retinal degenerative diseases.EMBO molecular medicine · 2018Review
- Cell culture: complications due to mechanical release of ATP and activation of purinoceptors.Cell and tissue research · 2017Review
- Oxytocin (OXT)-stimulated inhibition of Kir7.1 activity is through PIPCellular signalling · 2017Article
- Targeting the tight junction protein, zonula occludens-1, with the connexin43 mimetic peptide, αCT1, reduces VEGF-dependent RPE pathophysiology.Journal of molecular medicine (Berlin, Germany) · 2017Article
- Article
- Osmotic regulation ofMolecular vision · 2017Article
- Lack of the P2XBiochemical and biophysical research communications · 2017Article
- In Pursuit of Authenticity: Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium for Clinical Applications.Stem cells translational medicine · 2016Article
- Rat, mouse, and primate models of chronic glaucoma show sustained elevation of extracellular ATP and altered purinergic signaling in the posterior eye.Investigative ophthalmology & visual science · 2015Article
- Computational Model of Ca2+ Wave Propagation in Human Retinal Pigment Epithelial ARPE-19 Cells.PloS one · 2015Article
- Vesicular expression and release of ATP from dopaminergic neurons of the mouse retina and midbrain.Frontiers in cellular neuroscience · 2015Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
Abstract
1. Stimulation of purinergic receptors on retinal pigment epithelial (RPE) cells can increase the rate of fluid transport or decrease phagocytosis. This study aims to: determine whether the purine ATP can be released from RPE cells, begin probing the mechanism of any release and test whether cells degrade ATP extracellularly. 2. ATP release was monitored from cultured human ARPE-19 cells with the luciferin-luciferase assay. Biphasic release of ATP was triggered by basic fibroblast growth factor (bFGF), by the pyrimidine uridine triphosphate (UTP) and by hypotonicity. 3. The Cl(-) channel blocker 5-nitro-2-(3-phenylpropylamino)-benzoate (NPPB) inhibited release of ATP, suggesting that release was associated with Cl(-) channels. 4. Elevating intracellular Ca(2+) directly with ionomycin was insufficient to trigger ATP release. 5. UTP induced a biphasic elevation in intracellular Ca(2+). NPPB inhibited the second phase, suggesting autostimulation by released ATP. 6. Cells grown on permeable supports showed apical release of ATP, analogous to release into subretinal space in vivo. 7. The presence of ecto-ATPases on ARPE-19 cell membranes was suggested by the degradation of ATP added to intact cells. 8. Phagocytosis of fluorescent beads was inhibited by ATP, but the ecto-5'-nucleotidase inhibitor alpha, beta-methylene ADP prevented this, suggesting that inhibition was mediated by extracellular conversion of ATP to adenosine. 9. These results suggest that growth factors, pyrimidines and changes in tonicity could trigger ATP release into subretinal space. The levels of ATP released may be capable of autocrine stimulation of ATP receptors, while conversion to adenosine by ecto-enzymes could alter phagocytosis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.