ArticleThe Journal of physiology2001
K(+)-dependent gating of K(ir)1.1 channels is linked to pH gating through a conformational change in the pore.
Article in The Journal of physiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Characterization of hyperactive mutations in the renal potassium channel ROMK uncovers unique effects on channel biogenesis and ion conductance.Molecular biology of the cell · 2024Article
- Complementary computational and experimental evaluation of missense variants in the ROMK potassium channel.PLoS computational biology · 2020Article
- Direct injection of cell-free Kir1.1 protein into Xenopus oocytes replicates single-channel currents derived from Kir1.1 mRNA.Channels (Austin, Tex.) · 2015Article
- Molecular aspects of structure, gating, and physiology of pH-sensitive background K2P and Kir K+-transport channels.Physiological reviews · 2015Review
- Inhibition of ROMK channels by low extracellular K+ and oxidative stress.American journal of physiology. Renal physiology · 2013Article
- Residues at the outer mouth of Kir1.1 determine K-dependent gating.Biophysical journal · 2012Article
- Structural correlates of selectivity and inactivation in potassium channels.Biochimica et biophysica acta · 2012Review
- Potassium-dependent activation of Kir4.2 K⁺ channels.The Journal of physiology · 2011Article
- The pore structure and gating mechanism of K2P channels.The EMBO journal · 2011Article
- Functional and developmental expression of a zebrafish Kir1.1 (ROMK) potassium channel homologue Kcnj1.The Journal of physiology · 2011Article
- Modulation of Kir1.1 inactivation by extracellular Ca and Mg.Biophysical journal · 2011Article
- Structural basis for the coupling between activation and inactivation gates in K(+) channels.Nature · 2010Article
- An intersubunit salt bridge near the selectivity filter stabilizes the active state of Kir1.1.Biophysical journal · 2009Article
- Article
- External K activation of Kir1.1 depends on the pH gate.Biophysical journal · 2007Article
- Localization of the pH gate in Kir1.1 channels.Biophysical journal · 2006Article
- Structural and functional analysis of the putative pH sensor in the Kir1.1 (ROMK) potassium channel.EMBO reports · 2006Article
- Role of conserved glycines in pH gating of Kir1.1 (ROMK).Biophysical journal · 2006Article
- Interactive domains between pore loops of the yeast K+ channel TOK1 associate with extracellular K+ sensitivity.The Biochemical journal · 2006Article
- Structural locus of the pH gate in the Kir1.1 inward rectifier channel.Biophysical journal · 2005Article
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5 authors.
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Abstract
1. We have used giant patch-clamp recording to investigate the interaction between pH gating and K(+)-dependent gating in rat K(ir)1.1 (ROMK) channels heterologously expressed in Xenopus oocytes. 2. Gating by intracellular protons (pH gating) and extracellular K(+) ions (K(+)-dependent gating) is a hallmark of K(ir)1.1 channels that mediate K(+) secretion and control NaCl reabsorption in the kidney. pH gating is driven by protonation of an intracellular lysine residue (K80 in K(ir)1.1). K(+)-dependent gating occurs upon withdrawal of K(+) ions from the extracellular side of the channel. Both gating mechanisms are thought to interact allosterically. 3. K(+)-dependent gating was shown to be strictly coupled to pH gating; it only occurred when channels were in the pH-inactivated closed state, but not in the open state. Moreover, K(+)-dependent gating was absent in the non-pH-gated mutant K(ir)1.1(K80 M). 4. Channels inactivated by K(+)-dependent gating were reactivated upon addition of permeant ions to the extracellular side of the membrane, while impermeant ions failed to induce channel reactivation. Moreover, mutagenesis identified two residues in the P-helix (L136 and V140 in K(ir)1.1) that are crucial for K(+)-dependent gating. Replacement of these residues with the ones present in the non-K(+)-gated K(ir)2.1 abolished K(+)-dependent gating of K(ir)1.1 channels without affecting pH gating. 5. The results indicate that pH gating and K(+)-dependent gating are coupled to each other via structural rearrangements in the inner pore involving the P-helix.
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