ArticleThe EMBO journal2001
Vps45p stabilizes the syntaxin homologue Tlg2p and positively regulates SNARE complex formation.
Article in The EMBO journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed.
- VPS45 is required for both diffuse and tip growth ofFrontiers in plant science · 2023Article
- A distinct inner nuclear membrane proteome in Saccharomyces cerevisiae gametes.G3 (Bethesda, Md.) · 2021Article
- Article
- How we approach: Severe congenital neutropenia and myelofibrosis due to mutations in VPS45.Pediatric blood & cancer · 2019Review
- Endocytic trafficking factor VPS45 is essential for spatial regulation of lens fiber differentiation in zebrafish.Development (Cambridge, England) · 2018Article
- The Sec1/Munc18 (SM) protein Vps45 is involved in iron uptake, mitochondrial function and virulence in the pathogenic fungus Cryptococcus neoformans.PLoS pathogens · 2018Article
- Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.Current genetics · 2017Article
- Insulin stimulates syntaxin4 SNARE complex assembly via a novel regulatory mechanism.Molecular and cellular biology · 2014Article
- Munc18-2 and syntaxin 3 control distinct essential steps in mast cell degranulation.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- CAPS and Munc13: CATCHRs that SNARE Vesicles.Frontiers in endocrinology · 2013Review
- Sorting of GLUT4 into its insulin-sensitive store requires the Sec1/Munc18 protein mVps45.Molecular biology of the cell · 2013Article
- Arabidopsis Sec1/Munc18 protein SEC11 is a competitive and dynamic modulator of SNARE binding and SYP121-dependent vesicle traffic.The Plant cell · 2013Article
- Functional separation of endosomal fusion factors and the class C core vacuole/endosome tethering (CORVET) complex in endosome biogenesis.The Journal of biological chemistry · 2013Article
- Low-resolution solution structures of Munc18:Syntaxin protein complexes indicate an open binding mode driven by the Syntaxin N-peptide.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The Sec1/Munc18 protein Vps45 regulates cellular levels of its SNARE binding partners Tlg2 and Snc2 in Saccharomyces cerevisiae.PloS one · 2012Article
- Organization of SNAREs within the Golgi stack.Cold Spring Harbor perspectives in biology · 2011Review
- The COG complex interacts directly with Syntaxin 6 and positively regulates endosome-to-TGN retrograde transport.The Journal of cell biology · 2011Article
- Vesicle fusion probability is determined by the specific interactions of munc18.The Journal of biological chemistry · 2010Article
- Munc18-1 and syntaxin1: unraveling the interactions between the dynamic duo.Cellular and molecular neurobiology · 2010Article
- Insulin-regulated trafficking of GLUT4 requires ubiquitination.Traffic (Copenhagen, Denmark) · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sec1p-like/Munc-18 (SM) proteins bind to t-SNAREs and inhibit ternary complex formation. Paradoxically, the absence of SM proteins does not result in constitutive membrane fusion. Here, we show that in yeast cells lacking the SM protein Vps45p, the t-SNARE Tlg2p is down-regulated, to undetectable levels, by rapid proteasomal degradation. In the absence of Vps45p, Tlg2p can be stabilized through abolition of proteasome activity. Surprisingly, the stabilized Tlg2p was targeted to the correct intracellular location. However, the stabilized Tlg2p is non-functional and unable to bind its cognate SNARE binding partners, Tlg1p and Vti1p, in the absence of Vps45p. A truncation mutant lacking the first 230 residues of Tlg2p no longer bound Vps45p but was able to form complexes with Tlg1p and Vti1p in the absence of the SM protein. These data provide us with two valuable insights into the function of SM proteins. First, SM proteins act as chaperone-like molecules for their cognate t-SNAREs. Secondly, SM proteins play an essential role in the activation process allowing their cognate t-SNARE to participate in ternary complex formation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.