ArticleJournal of clinical pathology2001
Paraffin wax embedded muscle is suitable for the diagnosis of muscular dystrophy.
Article in Journal of clinical pathology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Diagnostic utility of inflammatory markers in formalin-fixed and paraffin-embedded muscle biopsies for idiopathic inflammatory myopathies.Histopathology · 2026Article
- Mutation spectrum analysis ofF1000Research · 2022Article
- Preclinical Evaluation of a Food-Derived Functional Ingredient to Address Skeletal Muscle Atrophy.Nutrients · 2020Article
- SU5416 does not attenuate early RV angiogenesis in the murine chronic hypoxia PH model.Respiratory research · 2019Article
- Insulin signaling as a potential natural killer cell checkpoint in fatty liver disease.Hepatology communications · 2018Article
- Immunohistochemistry of sarcolemmal membrane-associated proteins in formalin-fixed and paraffin-embedded skeletal muscle tissue: a promising tool for the diagnostic evaluation of common muscular dystrophies.Diagnostic pathology · 2017Article
- Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining.International journal of cell biology · 2016Review
- Article
- Do's and don'ts in the preparation of muscle cryosections for histological analysis.Journal of visualized experiments : JoVE · 2015Article
- Article
- Rapid fixation and immunofluorescent staining of cultured cells using microwave irradiation.Journal of histotechnology · 2011Article
- Microwave oven-based technique for immunofluorescent staining of paraffin-embedded tissues.Journal of molecular histology · 2008Article
- The effects of glucocorticoid therapy on the inflammatory and dendritic cells in muscular dystrophies.International journal of experimental pathology · 2006Article
- Paraffin wax embedded muscle is suitable for the diagnosis of muscular dystrophy.Journal of clinical pathology · 2002Article
- Validation of a simple, rapid, and economical technique for distinguishing type 1 and 2 fibres in fixed and frozen skeletal muscle.Journal of clinical pathology · 2002Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
aimAt present, the diagnosis of muscular dystrophy is made by means of immunohistochemistry on frozen sections. The aim of this study was to develop a sensitive and reproducible immunohistochemical method for use on formalin fixed, paraffin wax embedded sections for the demonstration of dystrophin associated proteins and other muscle associated antigens.
methodsAll the cases studied were from the files of the department of histopathology, Great Ormond Street Hospital for Children NHS Trust. Immunohistochemistry was performed on paraffin wax embedded sections with heat mediated antigen retrieval and overnight incubation with the antibodies at room temperature. Four different pretreatment buffers were tested in the attempt to optimise the immunostaining. Frozen sections were run in parallel for direct comparison.
resultsAll the antibodies except delta sarcoglycan gave strong, consistent immunostaining in paraffin wax embedded sections, comparable with the frozen sections. The most consistent results were obtained using citrate/EDTA as the pretreatment buffer.
conclusionA reliable and reproducible technique has been established, using a heat mediated citrate/EDTA buffer antigen retrieval method, which works well for most of the antibodies needed to make the diagnosis of muscular dystrophy in formalin fixed, paraffin wax embedded sections. This technique overcomes some of the inherent problems encountered using frozen muscle tissue and it could become a valuable tool for the diagnosis of muscular dystrophy.
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