ArticleProceedings of the National Academy of Sciences of the United States of America2001
Essential roles for four cytoplasmic intermediate filament proteins in Caenorhabditis elegans development.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 117 citations in OpenAlex.
- The Caenorhabditis elegans cuticle and precuticle: a model for studying dynamic apical extracellular matrices in vivo.Genetics · 2024Review
- A conserved protein tyrosine phosphatase, PTPN-22, functions in diverse developmental processes in C. elegans.PLoS genetics · 2024Article
- A conserved protein tyrosine phosphatase, PTPN-22, functions in diverse developmental processes inbioRxiv : the preprint server for biology · 2024Article
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- Terminal web and vesicle trafficking proteins mediate nematode single-cell tubulogenesis.The Journal of cell biology · 2020Article
- Intestinal intermediate filament polypeptides in C. elegans: Common and isotype-specific contributions to intestinal ultrastructure and function.Scientific reports · 2020Article
- Promiscuous Dimerization Between the Caenorhabditis elegans IF Proteins and a Hypothesis to Explain How Multiple IFs Persist Over Evolutionary Time.Journal of molecular evolution · 2019Article
- A tensile trilayered cytoskeletal endotube drives capillary-like lumenogenesis.The Journal of cell biology · 2019Article
- Tubular Excretory Canal Structure Depends on Intermediate Filaments EXC-2 and IFA-4 inGenetics · 2018Article
- Intermediate filament accumulation can stabilize microtubules inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Immuno-proteomic analysis of Trichinella spiralis, T. pseudospiralis, and T. papuae extracts recognized by human T. spiralis-infected sera.Parasitology research · 2018Article
- Review
- The tail domain is essential but the head domain dispensable for C. elegans intermediate filament IFA-2 function.PloS one · 2015Article
- Unusual ultrastructures of the Branchiostoma IF protein C2 containing heptads in the tail.Protoplasma · 2014Article
- Metformin promotes lifespan through mitohormesis via the peroxiredoxin PRDX-2.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Expression of Vimentin Intermediate Filament for Vascular Development in Olive Flounder (Paralichthys olivaceus).Development & reproduction · 2014Article
- Nebulin binding impedes mutant desmin filament assembly.Molecular biology of the cell · 2013Article
- The specificity of the interaction between αB-crystallin and desmin filaments and its impact on filament aggregation and cell viability.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2013Article
- The secretory pathway calcium ATPase PMR-1/SPCA1 has essential roles in cell migration during Caenorhabditis elegans embryonic development.PLoS genetics · 2013Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The structural proteins of the cytoplasmic intermediate filaments (IFs) arise in the nematode Caenorhabditis elegans from eight reported genes and an additional three genes now identified in the complete genome. With the use of double-stranded RNA interference (RNAi) for all 11 C. elegans genes encoding cytoplasmic IF proteins, we observe phenotypes for the five genes A1, A2, A3, B1, and C2. These range from embryonic lethality (B1) and embryonic/larval lethality (A3) to larval lethality (A1 and A2) and a mild dumpy phenotype of adults (C2). Phenotypes A2 and A3 involve displaced body muscles and paralysis. They probably arise by reduction of hypodermal IFs that participate in the transmission of force from the muscle cells to the cuticle. The B1 phenotype has multiple morphogenetic defects, and the A1 phenotype is arrested at the L1 stage. Thus, at least four IF genes are essential for C. elegans development. Their RNAi phenotypes are lethal defects due to silencing of single IF genes. In contrast to C. elegans, no IF genes have been identified in the complete Drosophila genome, posing the question of how Drosophila can compensate for the lack of these proteins, which are essential in mammals and C. elegans. We speculate that the lack of IF proteins in Drosophila can be viewed as cytoskeletal alteration in which, for instance, stable microtubules, often arranged as bundles, substitute for cytoplasmic IFs.
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