ArticleJournal of clinical microbiology2001
Detection of RTX toxin gene in Vibrio cholerae by PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed, 149 citations in OpenAlex.
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- Antibacterial action, proteolytic immunity, and in vivo activity of a Vibrio cholerae microcin.Cell host & microbe · 2024Article
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- Exploring the dynamics of toxigenic environmental Vibrio mimicus and its comparative analysis with Vibrio cholerae of the southern Gangetic delta.Archives of microbiology · 2022Article
- Molecular characterization ofIranian journal of microbiology · 2021Article
- First Experimental Evidence for the Presence of Potentially ToxicAntibiotics (Basel, Switzerland) · 2021Article
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- Origin and Dissemination of Altered El Tor Vibrio cholerae O1 Causing Cholera in Odisha, India: Two and Half Decade's View.Frontiers in microbiology · 2021Article
- Molecular detection and phylogenetic analysis ofNew microbes and new infections · 2020Article
- Virulence Pattern and Genomic Diversity of Vibrio cholerae O1 and O139 Strains Isolated From Clinical and Environmental Sources in India.Frontiers in microbiology · 2020Article
- Characterization of Vibrio cholerae isolates from freshwater sources in northwest Ohio.PloS one · 2020Article
- Environmental and Clinical Strains ofFrontiers in microbiology · 2019Article
- Comparison and Evaluation of the Molecular Typing Methods for ToxigenicFrontiers in microbiology · 2018Article
- Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V. cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139. The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae. Their role in the pathogenicity of the bacterium requires further investigation.
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