ArticleJournal of clinical microbiology2001
Development of a rapid and sensitive test for identification of major pathogens in bovine mastitis by PCR.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
64 citing papers in PubMed, 258 citations in OpenAlex.
- Colourimetric LAMP detection of Escherichia coli in bovine milk using a simplified DNA preparation method.Veterinary research communications · 2026Article
- Comparative study of recurrent mastitis caused by methicillin-resistant Staphylococcus aureus and non-MRSA S. aureus in Holstein-Fresian cows.The Journal of veterinary medical science · 2026Article
- Antimicrobial susceptibility, molecular characterization and multilocus sequence typing of Staphylococcus aureus isolates associated with subclinical mastitis in buffaloes (Bubalus bubalis) in Haryana, India.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Detection ofAnimals : an open access journal from MDPI · 2026Article
- Molecular Insights Into the Phylogenetic Diversity, Virulence Determinants, and Antimicrobial Resistance Pattern ofVeterinary medicine international · 2026Article
- Molecular characterization of Streptococcus agalactiae strains isolated from pregnant women.Scientific reports · 2025Article
- Article
- Molecular and phenotypic identification of bacterial species isolated from cows with mastitis from three regions of Poland.BMC veterinary research · 2024Article
- Basic concepts, recent advances, and future perspectives in the diagnosis of bovine mastitis.Journal of veterinary science · 2024Review
- A Study on the Stability and Antimicrobial Efficacy of a Newly Modeled Teat Dip Solution Containing Chlorhexidine.Veterinary sciences · 2023Article
- The Genetic Diversity and Antimicrobial Resistance of Pyogenic Pathogens Isolated from Porcine Lymph Nodes.Antibiotics (Basel, Switzerland) · 2023Article
- Molecular characterization ofJournal of advanced veterinary and animal research · 2023Article
- Detection of antimicrobial resistance genes in extended spectrum beta-lactamase-producingIranian journal of veterinary research · 2023Article
- Is AMR in Dairy Products a Threat to Human Health? An Updated Review on the Origin, Prevention, Treatment, and Economic Impacts of Subclinical Mastitis.Infection and drug resistance · 2023Review
- Diagnosing Intramammary Infection: Meta-Analysis and Mapping Review on Frequency and Udder Health Relevance of Microorganism Species Isolated from Bovine Milk Samples.Animals : an open access journal from MDPI · 2022Review
- Comparison of the Microbiome of Artisanal Homemade and Industrial Feta Cheese through Amplicon Sequencing and Shotgun Metagenomics.Microorganisms · 2022Article
- Prevalence and Molecular Characterization ofAnimals : an open access journal from MDPI · 2022Article
- Essential Oils from Indigenous Iranian Plants: A Natural Weapon vs. Multidrug-ResistantMicroorganisms · 2022Article
- Phenotypic and Molecular Characterization of Bovine Mastitis Milk Origin Bacteria and Linkage of Intramammary Infection With Milk Quality.Frontiers in veterinary science · 2022Article
- Virulence and antimicrobial resistance gene profiles of Staphylococcus aureus associated with clinical mastitis in cattle.PloS one · 2022Article
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bovine mastitis is the most important source of loss for the dairy industry. A rapid and specific test for the detection of the main pathogens of bovine mastitis is not actually available. Molecular probes reacting in PCR with bacterial DNA from bovine milk, providing direct and rapid detection of Escherichia coli, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus parauberis, and Streptococcus uberis, have been developed. Two sets of specific primers were designed for each of these microorganisms and appeared to discriminate close phylogenic bacterial species (e.g., S. agalactiae and S. dysgalactiae). In addition, two sets of universal primers were designed to react as positive controls with all major pathogens of bovine mastitis. The sensitivities of the test using S. aureus DNA extracted from milk with and without a pre-PCR enzymatic lysis step of bacterial cells were compared. The detection limit of the assay was 3.125 x 10(2) CFU/ml of milk when S. aureus DNA was extracted with the pre-PCR enzymatic step compared to 5 x 10(3) CFU/ml of milk in the absence of the pre-PCR enzymatic step. This latter threshold of sensitivity is still compatible with its use as an efficient tool of diagnosis in bovine mastitis, allowing the elimination of expensive reagents. The two PCR tests avoid cumbersome and lengthy cultivation steps, can be performed within hours, and are sensitive, specific, and reliable for the direct detection in milk of the six most prevalent bacteria causing bovine mastitis.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.