Evidence map›Paper›PMID 11427562›Full record

ArticleJournal of clinical microbiology2001

Standardization of antifungal susceptibility variables for a semiautomated methodology.

J L Rodríguez-Tudela, M Cuenca-Estrella, T M Díaz-Guerra, E Mellado

Abstract readEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed.

  1. Beyond roughness: Lateral surface architecture drives Candida albicans virulence on denture polymers.Dental materials : official publication of the Academy of Dental Materials · 2026
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  3. Voriconazole Activity AgainstMolecules (Basel, Switzerland) · 2026
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  5. An Agar-Water-Assisted ODJournal of fungi (Basel, Switzerland) · 2026
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  8. Central Carbon Metabolism inJournal of fungi (Basel, Switzerland) · 2024
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  15. Article
  16. A comparative GC-MS analysis of bioactive secondary metabolites produced by halotolerant Bacillus spp. isolated from the Great Sebkha of Oran.International microbiology : the official journal of the Spanish Society for Microbiology · 2021
    Article
  17. Article
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  19. Antimicrobial agents and chemotherapy · 2017
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

J L Rodríguez-TudelaServicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, 28220 Majadahonda, Spain. juanl.rodriguez-tudela@isciii.es
M Cuenca-Estrella
T M Díaz-Guerra
E Mellado

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Recently, the methodology that will serve as a basis of the standard for antifungal susceptibility testing of fermentative yeasts of the European Committee on Antibiotic Susceptibility Testing has been described. This procedure employs a spectrophotometric method for both inoculum adjustment and endpoint determination. However, the utilization of a spectrophotometer requires studies for standardization. The present work analyzes the following parameters: (i) accuracy of inoculum preparation, (ii) correlation between optical density and CFU per milliliter, (iii) influence of the wavelength on the endpoint determination, and (iv) influence of the dimethyl sulfoxide concentration on the growth kinetics. The main results can be summarized as follows: (i) inoculum preparation following the methodology recommended by the National Committee for Clinical Laboratory Standards is an exact procedure; (ii) the relationship between optical density and CFU per milliliter is linear (coefficient of determination, r(2) = 0.84); (iii) MICs obtained by means of spectrophotometric readings at different wavelengths are identical (for amphotericin B, an intraclass correlation coefficient of 0.98 was obtained; for fluconazole, the intraclass correlation coefficient was 1); and (iv) a 2% concentration of dimethyl sulfoxide produces a significantly slower and lower growth curve of Candida spp. than other concentrations.

Indexed as

Amphotericin BAntifungal AgentsCandidaCandidiasisColony Count, MicrobialDimethyl SulfoxideFluconazoleFungemiaHumansMicrobial Sensitivity TestsSpectrophotometryAmphotericin BAntifungal AgentsDimethyl SulfoxideFluconazole

Identifiers

PMID11427562
PMCPMC88178

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.