ArticleJournal of clinical microbiology2001
Serological expression cloning and immunological evaluation of MTB48, a novel Mycobacterium tuberculosis antigen.
Article in Journal of clinical microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Performance of purified antigens for serodiagnosis of pulmonary tuberculosis: a meta-analysis.Clinical and vaccine immunology : CVI · 2009Pooled it
- Antibodies as key mediators of protection againstFrontiers in immunology · 2024Review
- Antibodies as clinical tools for tuberculosis.Frontiers in immunology · 2023Review
- Priming mycobacterial ESX-secreted protein B to form a channel-like structure.Current research in structural biology · 2021Article
- Mycobacterium tuberculosis Secreted Proteins As Potential Biomarkers for the Diagnosis of Active Tuberculosis and Latent Tuberculosis Infection.Journal of clinical laboratory analysis · 2015Article
- Serum antibody responses to 10 Mycobacterium tuberculosis proteins, purified protein derivative, and old tuberculin in natural and experimental tuberculosis in rhesus monkeys.Clinical and vaccine immunology : CVI · 2011Article
- Identification of Mycobacterium tuberculosis antigens of high serodiagnostic value.Clinical and vaccine immunology : CVI · 2010Article
- Humoral immune responses against the Mycobacterium tuberculosis 38-kilodalton, MTB48, and CFP-10/ESAT-6 antigens in tuberculosis.Clinical and vaccine immunology : CVI · 2010Article
- Screening and assessing 11 Mycobacterium tuberculosis proteins as potential serodiagnostical markers for discriminating TB patients from BCG vaccinees.Genomics, proteomics & bioinformatics · 2009Article
- Evaluation of the Mtb72F polyprotein vaccine in a rabbit model of tuberculous meningitis.Infection and immunity · 2006Article
- ML0405 and ML2331 are antigens of Mycobacterium leprae with potential for diagnosis of leprosy.Clinical and vaccine immunology : CVI · 2006Article
- Assessing the serodiagnostic potential of 35 Mycobacterium tuberculosis proteins and identification of four novel serological antigens.Journal of clinical microbiology · 2005Article
- Epitope-specific antibody levels demonstrate recognition of new epitopes and changes in titer but not affinity during treatment of tuberculosis.Clinical and diagnostic laboratory immunology · 2004Article
- Potential serological use of a recombinant protein that is a replica of a Mycobacterium tuberculosis protein found in the urine of infected mice.Clinical and diagnostic laboratory immunology · 2004Article
- Comparison of antibody responses to a potential combination of specific glycolipids and proteins for test sensitivity improvement in tuberculosis serodiagnosis.Clinical and diagnostic laboratory immunology · 2004Article
- Human T-cell responses to the RD1-encoded protein TB27.4 (Rv3878) from Mycobacterium tuberculosis.Immunology · 2003Article
- PPE antigen Rv2430c of Mycobacterium tuberculosis induces a strong B-cell response.Infection and immunity · 2003Article
- Use of multiepitope polyproteins in serodiagnosis of active tuberculosis.Clinical and diagnostic laboratory immunology · 2002Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 2 countries.
Funding
Abstract
Improved diagnostics are needed for the detection of Mycobacterium tuberculosis, especially for patients with smear-negative disease. To address this problem, we have screened M. tuberculosis (H37Rv and Erdman strains) genomic expression libraries with pooled sera from patients with extrapulmonary disease and with sera from patients with elevated reactivity with M. tuberculosis lysate. Both serum pools were reactive with clones expressing a recombinant protein referred to here as MTB48. The genomic sequence of the resulting clones was identical to that of the M. tuberculosis H37Rv isolate and showed 99% identity to the Mycobacterium bovis and M. bovis BCG isolate sequences. The genomic location of this sequence is 826 bp upstream of a region containing the esat-6 gene that is deleted in the M. bovis BCG isolate. The mtb48 1,380-bp open reading frame encodes a predicted 47.6-kDa polypeptide with no known function. Southern and Western blot analyses indicate that this sequence is present in a single copy and is conserved in the M. tuberculosis and M. bovis isolates tested but not in other mycobacterial species tested, including Mycobacterium leprae and Mycobacterium avium. In addition, the native protein was detected in the cytoplasm, as was a processed form that was also shed into the medium during culture. Serological analysis of recombinant MTB48 and the M. tuberculosis 38-kDa antigen with a panel of patient and control sera indicates that the inclusion of recombinant MTB48 in a prototype serodiagnostic test increases assay sensitivity for M. tuberculosis infection when it is combined with other known immunodominant antigens, such as the 38-kDa antigen.
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