ArticleClinical and diagnostic laboratory immunology2001
AhpC, AhpD, and a secreted 14-kilodalton antigen from Mycobacterium avium subsp. paratuberculosis distinguish between paratuberculosis and bovine tuberculosis in an enzyme-linked immunosorbent assay.
Article in Clinical and diagnostic laboratory immunology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Specificity Evaluation of a Polyprotein-Based ELISA Designed for the Detection of Paratuberculosis in Multiple Species.Veterinary medicine international · 2026Article
- The Impact of Bacillus Calmette-Guérin Vaccination andVaccines · 2025Article
- Serological and epidemiological investigation of Mycobacterium avium subspecies paratuberculosis in bovines in Pakistan.Animal bioscience · 2024Article
- The Occurrence ofAnimals : an open access journal from MDPI · 2021Article
- Development and Validation of a Novel ELISA for the Specific Detection of Antibodies againstVeterinary medicine international · 2021Article
- Effect of Mycobacterium avium subsp. paratuberculosis (MAP) infection on the diagnostic accuracy for Mycobacterium bovis (M. bovis) infection under field conditions in cattle belonging to low M. bovis prevalence herds.Tropical animal health and production · 2017Article
- Composition and Potency Characterization of Mycobacterium avium subsp. paratuberculosis Purified Protein Derivatives.PloS one · 2016Article
- The Single Intradermal Cervical Comparative Test Interferes with Johne's Disease ELISA Diagnostics.Frontiers in immunology · 2014Article
- Production and proteomic characterisation of purified protein derivative from Mycobacterium avium subsp. paratuberculosis.Proteome science · 2012Article
- Occurrence of false-positive results in three paratuberculosis - ELISAs performed in a tuberculous herd.Veterinary research communications · 2009Article
- Profiling bovine antibody responses to Mycobacterium avium subsp. paratuberculosis infection by using protein arrays.Infection and immunity · 2008Article
- Mycobacterium avium in the postgenomic era.Clinical microbiology reviews · 2007Review
- Expression and immunogenicity of proteins encoded by sequences specific to Mycobacterium avium subsp. paratuberculosis.Journal of clinical microbiology · 2004Article
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sera from cattle naturally infected with Mycobacterium avium subsp. paratuberculosis (n = 56) and naturally (n = 4) and experimentally (n = 8) infected with Mycobacterium bovis were tested for the presence of antibodies against paratuberculosis antigens. An enzyme-linked immunosorbent assay (ELISA) was established based on absorption of M. avium subsp. paratuberculosis antigens on a hyperimmune antiserum against M. avium subsp. avium proteins in order to remove cross-reacting antigens. This absorbed-antigen ELISA recognized 66% of animals with paratuberculosis (37 of 56), while none of the animals with naturally occurring bovine tuberculosis (TB) had detectable antibodies. However, the animals with experimental bovine TB also responded in this ELISA. Similar results were found in a commercial ELISA, showing that neither of these tests was able to distinguish between paratuberculosis and bovine TB. The sera were further tested for antibody activities against purified AhpC and AhpD, which are proteins constitutively expressed by M. avium subsp. paratuberculosis, and against a secreted 14-kDa protein present in culture filtrates from the M. avium complex. Elevated antibody levels to AhpC, AhpD, and the 14-kDa antigen were found in 27% (13 of 48), 15% (7 of 48), and 27% (13 of 48), respectively, of the cattle with paratuberculosis. Together these ELISAs were positive with 35% (17 of 48) of the animals. None of the animals with bovine TB had detectable antibodies against any of the purified proteins despite their high levels of cross-reacting antibodies. These results show that purified specific antigens are needed to differentiate between paratuberculosis and bovine TB in ELISA.
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