ArticleApplied and environmental microbiology2001
Use of long-range repetitive element polymorphism-PCR to differentiate Bacillus anthracis strains.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Association of Genotyping of Bacillus cereus with Clinical Features of Post-Traumatic Endophthalmitis.PloS one · 2016Article
- Development of SCAR primers based on a repetitive DNA fingerprint for Escherichia coli detection.Journal of microbiology (Seoul, Korea) · 2013Article
- Existence of separate domains in lysin PlyG for recognizing Bacillus anthracis spores and vegetative cells.Antimicrobial agents and chemotherapy · 2012Article
- Determination of clonality and relatedness of Vibrio cholerae isolates by genomic fingerprinting, using long-range repetitive element sequence-based PCR.Applied and environmental microbiology · 2008Article
- Geographical distribution of genotypic and phenotypic markers among Bacillus anthracis isolates and related species by historical movement and horizontal transfer.Folia microbiologica · 2008Article
- Identification of Bacillus anthracis specific chromosomal sequences by suppressive subtractive hybridization.BMC genomics · 2004Article
- Quantitative oligonucleotide microarray fingerprinting of Salmonella enterica isolates.Nucleic acids research · 2004Article
- PCR assay of the groEL gene for detection and differentiation of Bacillus cereus group cells.Applied and environmental microbiology · 2003Article
- Bacillus anthracis diverges from related clades of the Bacillus cereus group in 16S-23S ribosomal DNA intergenic transcribed spacers containing tRNA genes.Applied and environmental microbiology · 2003Article
- Fingerprinting closely related xanthomonas pathovars with random nonamer oligonucleotide microarrays.Applied and environmental microbiology · 2002Article
- Utilization of the rpoB gene as a specific chromosomal marker for real-time PCR detection of Bacillus anthracis.Applied and environmental microbiology · 2001Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genome of Bacillus anthracis is extremely monomorphic, and thus individual strains have often proven to be recalcitrant to differentiation at the molecular level. Long-range repetitive element polymorphism-PCR (LR REP-PCR) was used to differentiate various B. anthracis strains. A single PCR primer derived from a repetitive DNA element was able to amplify variable segments of a bacterial genome as large as 10 kb. We were able to characterize five genetically distinct groups by examining 105 B. anthracis strains of diverse geographical origins. All B. anthracis strains produced fingerprints comprising seven to eight bands, referred to as "skeleton" bands, while one to three "diagnostic" bands differentiated between B. anthracis strains. LR REP-PCR fingerprints of B. anthracis strains showed very little in common with those of other closely related species such as B. cereus, B. thuringiensis, and B. mycoides, suggesting relative heterogeneity among the non-B. anthracis strains. Fingerprints from transitional non-B. anthracis strains, which possessed the B. anthracis chromosomal marker Ba813, scarcely resembled those observed for any of the five distinct B. anthracis groups that we have identified. The LR REP-PCR method described in this report provides a simple means of differentiating B. anthracis strains.
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