ArticleApplied and environmental microbiology2001
Study of genetic diversity of eukaryotic picoplankton in different oceanic regions by small-subunit rRNA gene cloning and sequencing.
Article in Applied and environmental microbiology, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 117 papers.
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117 citing papers in PubMed, 494 citations in OpenAlex.
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- Small Phytoplankton Community Composition Cycles Annually With a Coastal Bloom.Environmental microbiology · 2025Article
- Small Things that Make a Big Difference: Single-Cell Transcriptomic of Nanociliates Reveals Genes Potentially Involved in Mixotrophy.Microbial ecology · 2025Article
- How marine are Marine Stramenopiles (MAST)? A cross-system evaluation.FEMS microbiology ecology · 2024Article
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- Contrasting Mechanisms Determine the Microeukaryotic and Syndiniales Community Assembly in a Eutrophic bay.Microbial ecology · 2023Article
- Capturing marine microbiomes and environmental DNA: A field sampling guide.Frontiers in microbiology · 2022Review
- Comparison of Deep-Sea Picoeukaryotic Composition Estimated from the V4 and V9 Regions of 18S rRNA Gene with a Focus on the Hadal Zone of the Mariana Trench.Microbial ecology · 2022Article
- Comparative analysis of freshwater phytoplankton communities in two lakes of Burabay National Park using morphological and molecular approaches.Scientific reports · 2021Article
- A decadal perspective on north water microbial eukaryotes as Arctic Ocean sentinels.Scientific reports · 2021Article
- Annual phytoplankton dynamics in coastal waters from Fildes Bay, Western Antarctic Peninsula.Scientific reports · 2021Article
- Eukaryotic Cell Capture by Amplified MagneticFrontiers in microbiology · 2021Article
- Influence of Regional Oceanography and Hydrothermal Activity on Protist Diversity and Community Structure in the Okinawa Trough.Microbial ecology · 2020Article
- Nitrate Consumers in Arctic Marine Eukaryotic Communities: Comparative Diversities of 18S rRNA, 18S rRNA Genes, and Nitrate Reductase Genes.Applied and environmental microbiology · 2019Article
- Single cell genomics yields a wide diversity of small planktonic protists across major ocean ecosystems.Scientific reports · 2019Article
- Time series are critical to understand microbial plankton diversity and ecology.Molecular ecology · 2019Article
- Assessing eukaryotic biodiversity in the Florida Keys National Marine Sanctuary through environmental DNA metabarcoding.Ecology and evolution · 2019Article
- Diversity and Spatial Distribution of Chromophytic Phytoplankton in the Bay of Bengal Revealed by RuBisCO Genes (Frontiers in microbiology · 2019Article
- Elucidating Viral Communities During a Phytoplankton Bloom on the West Antarctic Peninsula.Frontiers in microbiology · 2019Article
57 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Very small eukaryotic organisms (picoeukaryotes) are fundamental components of marine planktonic systems, often accounting for a significant fraction of the biomass and activity in a system. Their identity, however, has remained elusive, since the small cells lack morphological features for identification. We determined the diversity of marine picoeukaryotes by sequencing cloned 18S rRNA genes in five genetic libraries from North Atlantic, Southern Ocean, and Mediterranean Sea surface waters. Picoplankton were obtained by filter size fractionation, a step that excluded most large eukaryotes and recovered most picoeukaryotes. Genetic libraries of eukaryotic ribosomal DNA were screened by restriction fragment length polymorphism analysis, and at least one clone of each operational taxonomic unit (OTU) was partially sequenced. In general, the phylogenetic diversity in each library was rather great, and each library included many different OTUs and members of very distantly related phylogenetic groups. Of 225 eukaryotic clones, 126 were affiliated with algal classes, especially the Prasinophyceae, the Prymnesiophyceae, the Bacillariophyceae, and the Dinophyceae. A minor fraction (27 clones) was affiliated with clearly heterotrophic organisms, such as ciliates, the chrysomonad Paraphysomonas, cercomonads, and fungi. There were two relatively abundant novel lineages, novel stramenopiles (53 clones) and novel alveolates (19 clones). These lineages are very different from any organism that has been isolated, suggesting that there are previously unknown picoeukaryotes. Prasinophytes and novel stramenopile clones were very abundant in all of the libraries analyzed. These findings underscore the importance of attempts to grow the small eukaryotic plankton in pure culture.
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