ArticleRNA (New York, N.Y.)2001
The mechanism and regulation of deadenylation: identification and characterization of Xenopus PARN.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
42 citing papers in PubMed, 109 citations in OpenAlex.
- Landscape and regulation of mRNA translation in the early C. elegans embryo.Cell reports · 2025Article
- Landscape and regulation of mRNA translation in the earlybioRxiv : the preprint server for biology · 2025Article
- Recycling of Uridylated mRNAs in Starfish Embryos.Biomolecules · 2024Article
- A genome-wide perspective of the maternal mRNA translation program during oocyte development.Seminars in cell & developmental biology · 2024Review
- Poly (A)-specific ribonuclease deficiency impacts oogenesis in zebrafish.Scientific reports · 2023Article
- Identification of PARN nuclease activity inhibitors by computational-based docking and high-throughput screening.Scientific reports · 2023Article
- Regulation of poly(a)-specific ribonuclease activity by reversible lysine acetylation.The Journal of biological chemistry · 2020Article
- Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption.Nucleic acids research · 2020Article
- Regulation of Germ Cell mRNPs by eIF4E:4EIP Complexes: Multiple Mechanisms, One Goal.Frontiers in cell and developmental biology · 2020Review
- Article
- Expanding the repertoire of deadenylases.RNA biology · 2017Review
- A feedback mechanism between PLD and deadenylase PARN for the shortening of eukaryotic poly(A) mRNA tails that is deregulated in cancer cells.Biology open · 2017Article
- FXR1a-associated microRNP: A driver of specialized non-canonical translation in quiescent conditions.RNA biology · 2017Review
- Translational Control of Germ Cell Decisions.Results and problems in cell differentiation · 2017Review
- Hormonal stimulation of starfish oocytes induces partial degradation of the 3' termini of cyclin B mRNAs with oligo(U) tails, followed by poly(A) elongation.RNA (New York, N.Y.) · 2016Article
- The RNase PARN-1 Trims piRNA 3' Ends to Promote Transcriptome Surveillance in C. elegans.Cell · 2016Article
- PARN deadenylase is involved in miRNA-dependent degradation of TP53 mRNA in mammalian cells.Nucleic acids research · 2015Article
- Maturation of mammalian H/ACA box snoRNAs: PAPD5-dependent adenylation and PARN-dependent trimming.RNA (New York, N.Y.) · 2012Article
- An integrated in silico approach to design specific inhibitors targeting human poly(a)-specific ribonuclease.PloS one · 2012Article
- Translational control in oocyte development.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
In Xenopus oocytes, the deadenylation of a specific class of maternal mRNAs results in their translational repression. Here we report the purification, characterization, and molecular cloning of the Xenopus poly(A) ribonuclease (xPARN). xPARN copurifies with two polypeptides of 62 kDa and 74 kDa, and we provide evidence that the 62-kDa protein is a proteolytic product of the 74-kDa protein. We have isolated the full-length xPARN cDNA, which contains the tripartite exonuclease domain conserved among RNase D family members, a putative RNA recognition motif, and a domain found in minichromosome maintenance proteins. Characterization of the xPARN enzyme shows that it is a poly(A)-specific 3' exonuclease but does not require an A residue at the 3' end. However, the addition of 25 nonadenylate residues at the 3' terminus, or a 3' terminal phosphate is inhibitory. Western analysis shows that xPARN is expressed throughout early development, suggesting that it may participate in the translational silencing and destabilization of maternal mRNAs during both oocyte maturation and embryogenesis. In addition, microinjection experiments demonstrate that xPARN can be activated in the oocyte nucleus in the absence of cytoplasmic components and that nuclear export of deadenylated RNA is impeded. Based on the poly(A) binding activity of xPARN in the absence of catalysis, a model for substrate specificity is proposed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.