ArticleOncogene2001
The synovial sarcoma associated protein SYT interacts with the acute leukemia associated protein AF10.
Article in Oncogene, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 48 citations in OpenAlex.
- Synovial Sarcoma: A Complex Disease with Multifaceted Signaling and Epigenetic Landscapes.Current oncology reports · 2020Review
- Cystic synovial sarcoma of the pleura mimicking a cystic thymoma: a case report illustrating the role of decreased INI-1 expression in differential diagnosis.International journal of clinical and experimental pathology · 2015Article
- Truncated SSX protein suppresses synovial sarcoma cell proliferation by inhibiting the localization of SS18-SSX fusion protein.PloS one · 2013Article
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- The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties.Plant physiology · 2009Article
- Cytosolic SYT/SS18 isoforms are actin-associated proteins that function in matrix-specific adhesion.PloS one · 2009Article
- The proto-oncoprotein SYT (SS18) controls ATP release and regulates cyst formation by polarized MDCK cells.Experimental cell research · 2008Article
- Synovial sarcoma: from genetics to genetic-based animal modeling.Clinical orthopaedics and related research · 2008Review
- The C terminus of the synovial sarcoma-associated SSX proteins interacts with the LIM homeobox protein LHX4.Oncogene · 2008Article
- The SYT-SSX fusion protein down-regulates the cell proliferation regulator COM1 in t(x;18) synovial sarcoma.Molecular and cellular biology · 2007Article
- The robotic mouse: unravelling the function of AF4 in the cerebellum.Cerebellum (London, England) · 2005Review
- MLL fusion partners AF4 and AF9 interact at subnuclear foci.Leukemia · 2004Article
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Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As a result of the synovial sarcoma associated t(X;18) translocation, the human SYT gene on chromosome 18 is fused to either the SSX1 or the SSX2 gene on the X chromosome. Although preliminary evidence indicates that the (fusion) proteins encoded by these genes may play a role in transcriptional regulation, little is known about their exact function. We set out to isolate interacting proteins through yeast two hybrid screening of a human cDNA library using SYT as a bait. Of the positive clones isolated, two were found to correspond to the acute leukemia t(10;11) associated AF10 gene, a fusion partner of MLL. Confirmation of these results was obtained via co-immunoprecipitation of endogenous and exogenous, epitope-tagged, SYT and AF10 proteins from cell line extracts and colocalization of epitope-tagged SYT and AF10 proteins in transfected cells. Subsequent sequential mutation analysis revealed a highly specific interaction of N-terminal SYT fragments with C-terminal AF10 fragments. The N-terminal interaction domain of the SYT protein was also found to be present in several SYT orthologs and homologs. The C-terminal interaction domain of AF10 is located outside known functional domains. Based on these results, a model is proposed in which the SYT and AF10 proteins act in concert as bipartite transcription factors. This model has implications for the molecular mechanisms underlying the development of both human synovial sarcomas and acute leukemias.
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