ArticleBiophysical journal2001
Membrane stress and permeabilization induced by asymmetric incorporation of compounds.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 81 citations in OpenAlex.
- Flip Flop-Associated Growth and Division of Synthetic Cells.ACS nano · 2026Article
- Quantifying Small-Molecule Association with Lipid Membranes: Methods, Models, and Limitations.Membranes · 2026Review
- Strong Membrane Permeabilization Activity Can Reduce Selectivity of Cyclic Antimicrobial Peptides.The journal of physical chemistry. B · 2025Article
- Interaction of the Antibiotic Rifampicin with Lipid Membranes.Biomolecules · 2025Article
- Melittin can permeabilize membranes via large transient pores.Nature communications · 2024Article
- Controlled node growth on the surface of polymersomes.Chemical science · 2024Article
- Vesicle budding caused by lysolipid-induced asymmetry stress.Biophysical journal · 2023Article
- An Experimental Approach to Address the Functional Relationship between Antioxidant Enzymes and Mitochondrial Respiratory Complexes.Methods and protocols · 2023Article
- Interactions of Bacterial Quorum Sensing Signals with Model Lipid Membranes: Influence of Membrane Composition on Membrane Remodeling.Langmuir : the ACS journal of surfaces and colloids · 2023Article
- Interaction of a Homologous Series of Amphiphiles with P-glycoprotein in a Membrane Environment-Contributions of Polar and Non-Polar Interactions.Pharmaceutics · 2023Article
- Leaky membrane fusion: an ambivalent effect induced by antimicrobial polycations.Nanoscale advances · 2022Article
- Effects of cholesterol on the mechanism of fengycin, a biofungicide.Biophysical journal · 2022Article
- The effect of membrane thickness on the membrane permeabilizing activity of the cyclic lipopeptide tolaasin II.Frontiers in molecular biosciences · 2022Article
- Interactions of Bacterial Quorum Sensing Signals with Model Lipid Membranes: Influence of Acyl Tail Structure on Multiscale Response.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- The Optimal Lipid Chain Length of a Membrane-Permeabilizing Lipopeptide Results From the Balance of Membrane Partitioning and Local Damage.Frontiers in microbiology · 2021Article
- Lipid Scrambling Induced by Membrane-Active Substances.Biophysical journal · 2020Article
- The Mechanism of Membrane Permeabilization by Peptides: Still an Enigma.Australian journal of chemistry · 2019Article
- NH125 kills methicillin-resistant Staphylococcus aureus persisters by lipid bilayer disruption.Future medicinal chemistry · 2016Article
- Correlating antimicrobial activity and model membrane leakage induced by nylon-3 polymers and detergents.Soft matter · 2015Article
- Real-time monitoring of membrane-protein reconstitution by isothermal titration calorimetry.Analytical chemistry · 2014Article
Corrections and comments
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Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The area balance or imbalance between the inner and outer monolayer of biological membranes is a key parameter for driving shape changes (including exo and endocytosis) and controlling the bilayer curvature stress. The asymmetric incorporation of a drug or biological agent interferes with these processes, and the subsequent stress may lead to a membrane permeation or permeabilization. A main goal of this study is to introduce new methods to characterize such phenomena using isothermal titration calorimetry. POPC unilamellar vesicles and a series of alkyl maltosides are used as model systems; the unilamellarity was checked by NMR with the shift reagent Pr(3+). The free energy, enthalpy, and entropy associated with the asymmetry stress are estimated by comparing partitioning data of uptake versus release assays. The asymmetry stress is of enthalpic nature and somewhat reduced by entropic effects. Stimulated membrane permeation occurs at a mean maltoside-to-lipid ratio of approximately 0.2, which corresponds to an apparent area asymmetry of approximately 30% and a limiting free energy of the order of 2 kJ/mol of maltoside. Membrane solubilization to coexisting micelles proceeds at mole ratios of approximately 0.73, 0.81, and 0.88 (C(12)-, C(13)-, and C(14)-maltoside, respectively). Experiments with vesicles pre-loaded with surfactant in both monolayers provide evidence that the translocation threshold is controlled by the asymmetrically incorporated surfactant, whereas the onset of solubilization depends on the total surfactant content in the membrane. Free copies of the uptake and release fitting script including instructions are available upon request to heerklotz@gmx.net.
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