ArticleBiophysical journal2001
Direct measurement of the area expansion and shear moduli of the human red blood cell membrane skeleton.
Article in Biophysical journal, 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed.
- Red blood cell deformation in a microfluidic sudden expansion at supraphysiological strain rates.Biophysical journal · 2026Article
- Modeling Red Blood Cell Deformation at Supraphysiological Strain Rates Using a Droplet Framework.Annals of biomedical engineering · 2026Article
- Quantifying Experimental Variability in Shear-Induced Hemolysis to Support Uncertainty-Aware Hemolysis Models.Annals of biomedical engineering · 2025Article
- Pathophysiological Associations and Measurement Techniques of Red Blood Cell Deformability.Biosensors · 2025Review
- Stomatocyte-discocyte-echinocyte transformations of erythrocyte modulated by membrane-cytoskeleton mechanical properties.Biophysical journal · 2025Article
- Electro-deformation spectroscopy: A unified method for simultaneous electrical and mechanical characterization of single cells.Acta biomaterialia · 2025Article
- Red blood cell flickering activity locally controlled by holographic optical tweezers.iScience · 2024Article
- Effective cell membrane tension protects red blood cells against malaria invasion.PLoS computational biology · 2023Article
- Measurement of red blood cell deformability during morphological changes using rotating-glass-plate-based scanning optical tweezers.Biomedical optics express · 2023Article
- Energy Dissipation in the Human Red Cell Membrane.Biomolecules · 2023Article
- Mechanical properties of external confinement modulate the rounding dynamics of cells.Biophysical journal · 2021Article
- Constitutive Model of Erythrocyte Membranes with Distributions of Spectrin Orientations and Lengths.Biophysical journal · 2020Article
- Review
- Optical Tweezers in Studies of Red Blood Cells.Cells · 2020Article
- DNA cytoskeleton for stabilizing artificial cells.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Mechanical diagnosis of human erythrocytes by ultra-high speed manipulation unraveled critical time window for global cytoskeletal remodeling.Scientific reports · 2017Article
- Probing cytoskeletal pre-stress and nuclear mechanics in endothelial cells with spatiotemporally controlled (de-)adhesion kinetics on micropatterned substrates.Cell adhesion & migration · 2017Article
- Large Deformation Properties of Red Blood Cell Membrane Based on a Higher Order Gradient Quasi-continuum Model.The Journal of membrane biology · 2015Article
- Stiffening of Red Blood Cells Induced by Cytoskeleton Disorders: A Joint Theory-Experiment Study.Biophysical journal · 2015Article
- Stretching of red blood cells using an electro-optics trap.Biomedical optics express · 2015Article
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5 authors.
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Abstract
The area expansion and the shear moduli of the free spectrin skeleton, freshly extracted from the membrane of a human red blood cell (RBC), are measured by using optical tweezers micromanipulation. An RBC is trapped by three silica beads bound to its membrane. After extraction, the skeleton is deformed by applying calibrated forces to the beads. The area expansion modulus K(C) and shear modulus mu(C) of the two-dimensional spectrin network are inferred from the deformations measured as functions of the applied stress. In low hypotonic buffer (25 mOsm/kg), one finds K(C) = 4.8 +/- 2.7 microN/m, mu(C) = 2.4 +/- 0.7 microN/m, and K(C)/mu(C) = 1.9 +/- 1.0. In isotonic buffer, one measures higher values for K(C), mu(C), and K(C)/mu(C), partly because the skeleton collapses in a high-ionic-strength environment. Some data concerning the time evolution of the mechanical properties of the skeleton after extraction and the influence of ATP are also reported. In the Discussion, it is shown that the measured values are consistent with estimates deduced from experiments carried out on the intact membrane and agree with theoretical and numerical predictions concerning two-dimensional networks of entropic springs.
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