ArticleRNA (New York, N.Y.)2001
A purine-rich intronic element enhances alternative splicing of thyroid hormone receptor mRNA.
Article in RNA (New York, N.Y.), 2001. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- TRα2-An Untuned Second Fiddle or Fine-Tuning Thyroid Hormone Action?International journal of molecular sciences · 2022Review
- Srsf10 and the minor spliceosome control tissue-specific and dynamic SR protein expression.eLife · 2020Article
- Human IL12RB1 expression is allele-biased and produces a novel IL12 response regulator.Genes and immunity · 2019Article
- Adaptive Gene Loss? Tracing Back the Pseudogenization of the Rabbit CCL8 Chemokine.Journal of molecular evolution · 2016Article
- TDP-43 functions within a network of hnRNP proteins to inhibit the production of a truncated human SORT1 receptor.Human molecular genetics · 2016Article
- Identification of Cryptic Novel α-Galactosidase A Gene Mutations: Abnormal mRNA Splicing and Large Deletions.JIMD reports · 2016Article
- Article
- Regulation of a novel isoform of Receptor Expression Enhancing Protein REEP6 in rod photoreceptors by bZIP transcription factor NRL.Human molecular genetics · 2014Article
- Article
- Pressure-overload cardiac hypertrophy is associated with distinct alternative splicing due to altered expression of splicing factors.Molecules and cells · 2014Article
- An intronic G run within HIV-1 intron 2 is critical for splicing regulation of vif mRNA.Journal of virology · 2013Article
- The high kinetic stability of a G-quadruplex limits hnRNP F qRRM3 binding to G-tract RNA.Nucleic acids research · 2013Article
- Article
- Alternative splicing of a single transcription factor drives selfish reproductive behavior in honeybee workers (Apis mellifera).Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site.The Journal of biological chemistry · 2011Article
- Structure and expression of two nuclear receptor genes in marsupials: insights into the evolution of the antisense overlap between the α-thyroid hormone receptor and Rev-erbα.BMC molecular biology · 2010Article
- A network of conserved co-occurring motifs for the regulation of alternative splicing.Nucleic acids research · 2010Article
- In vivo detection of RNA-binding protein interactions with cognate RNA sequences by fluorescence resonance energy transfer.RNA (New York, N.Y.) · 2009Article
- Splice site strength-dependent activity and genetic buffering by poly-G runs.Nature structural & molecular biology · 2009Article
- A computational approach for genome-wide mapping of splicing factor binding sites.Genome biology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The mammalian thyroid hormone receptor gene c-erbAalpha gives rise to two mRNAs that code for distinct isoforms, TRalpha1 and TRalpha2, with antagonistic functions. Alternative processing of these mRNAs involves the mutually exclusive use of a TRalpha1-specific polyadenylation site or TRalpha2-specific 5' splice site. A previous investigation of TRalpha minigene expression defined a critical role for the TRalpha2 5' splice site in directing alternative processing. Mutational analysis reported here shows that purine residues within a highly conserved intronic element, SEa2, enhance splicing of TRalpha2 in vitro as well as in vivo. Although SEalpha2 is located within the intron of TRalpha2 mRNA, it activates splicing of a heterologous dsx pre-mRNA when located in the downstream exon. Competition with wild-type and mutant RNAs indicates that SEalpha2 functions by binding trans-acting factors in HeLa nuclear extract. Protein-RNA crosslinking identifies several proteins, including SF2/ASF and hnRNP H, that bind specifically to SEalpha2. SEalpha2 also includes an element resembling a 5' splice site consensus sequence that is critical for splicing enhancer activity. Mutations within this pseudo-5' splice site sequence have a dramatic effect on splicing and protein binding. Thus SEa2 and its associated factors are required for splicing of TRalpha2 pre-mRNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.